Overview

  • Product name

    Mouse TCA-3 ELISA Kit (CCL1)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Intra-assay < 10%
    Inter-assay
    Sample n Mean SD CV%
    Inter-assay < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 30 pg/ml
  • Range

    0.033 ng/ml - 8 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 91.6 77% - 103%
    Serum 127.7 119% - 135%
    Plasma 126.3 109% - 139%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s TCA-3 (CCL1) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of TCA-3 (CCL1) in mouse serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse TCA-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and TCA-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TCA-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of TCA-3 bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    5X Assay Diluent D 1 x 15ml
    700X HRP-Streptavidin Concentrate 1 x 200µl
    Biotinylated anti-mouse TCA-3 2 vials
    Recombinant Mouse TCA-3 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TCA-3 Microplate (12 x 8 wells) 1 unit
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Alternative names

    • C-C motif chemokine 1
    • CCL1
    • chemokine (C-C motif) ligand 1
    • I-309
    • inflammatory cytokine I-309
    • P500
    • SCYA1
    • SISe
    • small inducible cytokine A1 (I-309, homologous to mouse Tca-3)
    • small-inducible cytokine A1
    • T lymphocyte-secreted protein I-309
    • TCA3
    see all

Applications

Our Abpromise guarantee covers the use of ab155460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155460 - Assay Diluent B.

  • Representative standard curve using ab155460 - Assay Diluent D.

Protocols

References

ab155460 has not yet been referenced specifically in any publications.

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