Key features and details
- Sensitivity: 30 pg/ml
- Range: 0.033 ng/ml - 8 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse TCA-3 ELISA Kit (CCL1)
Intra-assay Sample n Mean SD CV% Intra-assay < 10% Inter-assay Sample n Mean SD CV% Inter-assay < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 30 pg/ml
Range0.033 ng/ml - 8 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 91.6 77% - 103% Serum 127.7 119% - 135% Plasma 126.3 109% - 139%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s TCA-3 (CCL1) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of TCA-3 (CCL1) in mouse serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse TCA-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and TCA-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TCA-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of TCA-3 bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-mouse TCA-3 2 vials Recombinant Mouse TCA-3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TCA-3 Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml
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ab155460 has not yet been referenced specifically in any publications.