Product nameMouse TCS Antibody Purification Kit
See all Antibody Purification kits
Efficient mouse antibody purification from tissue culture supernatants. Processes 10-25 mL of supernatant to purify up to 1.5 mg of antibody per run.
The mouse resin has a very high affinity and specificity for mouse IgG molecules. ab128749 Mouse TCS Antibody Purification Kit can be used to purify mouse IgG fractions from hybridoma supernatants. The binding strength of bovine IgG to Abcam's Mouse resin is negligible; therefore, it can be used to selectively recover mouse IgG from TCS samples.
The method involves capture of the mouse antibody on the Mouse resin and removal of unwanted substances using a simple wash procedure. The purified product is eluted and neutralized.
Antibodies purified using the Mouse TCS Antibody Purification kit are fully compatible with our Lightning-Link® Antibody Conjugation Kits and our Oligonucleotide Conjugation Kit, however they are not compatible with our nanoparticle Antibody conjugation kits. To purify antibodies for use with our nanoparticle conjugation kits (Gold, Latex, Europium and Magnetic), please use our Mouse Antibody Purification Kit - Nanoparticles ab204910.
ab128749 is suitable for use with Mouse antibodies. It is not suitable for use with antibodies from other species.
This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ Mouse TCS Antibody Purification System. 832-0500 is the same as the 3 tests size. 832-0005 is the same as the 1 test size.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 tests 3 tests ab274298 - Binding Buffer (10X) 1 x 6ml 1 x 18ml ab274299 - Elution Buffer 1 x 5ml 1 x 15ml ab273960 - Mouse Resin 1 x 2ml 3 x 2ml ab274174 - Neutralizer Buffer 1 x 2ml 1 x 5ml ab274180 - Purification Column 1 unit 3 units ab273914 - Spin Cartridge / Collecting Tube Assemblies 1 unit 3 units ab274173 - Wash Buffer 1 x 25ml 1 x 75ml
A representative bar graph comparing the effectiveness of Mouse TCS Antibody Purification Kit and Protein G resin.
Gel electrophoresis of TCS samples purified with the Mouse TCS Antibody Purification Kit.
M = Mouse IgG1 in a TCS sample
B = Bovine serum IgG in a TCS sample
TCS = Tissue culture supernatant sample
ab128749 has been referenced in 4 publications.
- Melandri D et al. The ?dTCR combines innate immunity with adaptive immunity by utilizing spatially distinct regions for agonist selection and antigen responsiveness. Nat Immunol 19:1352-1365 (2018). PubMed: 30420626
- Tyc KM et al. The Conserved Intron Binding Protein EMB-4 Plays Differential Roles in Germline Small RNA Pathways of C. elegans. Dev Cell 42:256-270.e6 (2017). PubMed: 28787592
- Di Marco Barros R et al. Epithelia Use Butyrophilin-like Molecules to Shape Organ-Specific ?d T Cell Compartments. Cell 167:203-218.e17 (2016). PubMed: 27641500
- Crawford JR et al. Plasma Levels of Endothelial Microparticles Bearing Monomeric C-reactive Protein are Increased in Peripheral Artery Disease. J Cardiovasc Transl Res 9:184-93 (2016). PubMed: 26891844