Overview

  • Product name

    Mouse TFPI Matched Antibody Pair Kit
    See all TFPI kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse TFPI.


    Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.



    • Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.

    • Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.

    • Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.


    Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab217776) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Mouse TFPI Capture Antibody 1 x 100µg 1 x 20µg
    Mouse TFPI Detector Antibody 1 x 25µg 1 x 5µg
    Mouse TFPI Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
  • Tissue specificity

    Mostly in endothelial cells.
  • Sequence similarities

    Contains 3 BPTI/Kunitz inhibitor domains.
  • Domain

    This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Anti convertin
    • EPI
    • Extrinsic pathway inhibitor
    • LACI
    • Lipoprotein associated coagulation inhibitor
    • Lipoprotein-associated coagulation inhibitor
    • TFI
    • TFPI
    • TFPI 1
    • TFPI1
    • TFPI1_HUMAN
    • Tissue factor pathway inhibitor
    • Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor)
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab222259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab222259 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222259.
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