Overview

  • Product name

    Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT)
    See all Thrombin-Antithrombin Complex kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.8%
  • Sample type

    Cell culture supernatant, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    > 0.01 ng/ml
  • Range

    0.031 ng/ml - 8 ng/ml
  • Recovery

    97 %

  • Assay time

    5h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    TAT Complexes Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TAT Complexes levels in plasma and cell culture supernatants.


    A TAT Complexes specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TAT Complexes specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TAT Complexes captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Mouse TAT Complexes Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
    TAT Complexes Microplate (12 x 8 well strips) 1 unit
    TAT Complexes Standard 1 x 2 vials
  • Research areas

  • Relevance

    Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Antithrombin: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. Antithrombin inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Cellular localization

    Secreted
  • Alternative names

    • antithrombin III
    • AT3
    • AT3D
    • ATIII
    • coagulation factor II (thrombin)
    • F2
    • PRO0309
    • prothrombin
    • prothrombin B chain
    • PT
    • RPRGL2
    • serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
    • serine protease
    • serine-cysteine proteinase inhibitor clade C member 1
    • serpin C1
    • serpin peptidase inhibitor, clade C (antithrombin), member 1
    • SERPINC1
    • TAT
    • THPH1
    • THPH7
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab137994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse TAT measured in murine biological fluids (diluted 1/256; duplicates +/- SD).

  • Representative Standard Curve using ab137994

References

This product has been referenced in:

  • Ainsworth S  et al. The paraspecific neutralisation of snake venom induced coagulopathy by antivenoms. Commun Biol 1:34 (2018). Read more (PubMed: 30271920) »
  • Hayashi H  et al. Sirt3 deficiency does not affect venous thrombosis or NETosis despite mild elevation of intracellular ROS in platelets and neutrophils in mice. PLoS One 12:e0188341 (2017). Read more (PubMed: 29236713) »
See all 3 Publications for this product

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