Overview

  • Product name

    Mouse Thrombomodulin ELISA Kit
    See all Thrombomodulin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    mouse serum 8 2.5%
    Inter-assay
    Sample n Mean SD CV%
    mouse serum 3 8.3%
  • Sample type

    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    17.5 pg/ml
  • Range

    93.75 pg/ml - 6000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 104 98% - 115%
    Serum 105 101% - 107%
    Cell culture media 94 91% - 98%
    Heparin Plasma 92 86% - 99%
    EDTA Plasma 101 97% - 103%
    Citrate Plasma 102 93% - 115%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Cow, Human
  • Product overview

    Abcam’s mouse Thrombomodulin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombomodulin protein in serum, plasma, and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


     

  • Notes

    Mouse Thrombomodulin, which is encoded by the THBD gene, is an endothelial cell membrane protein that inhibits coagulation. Specifically, Thrombomodulin forms a 1:1 complex with thrombin, and this complex converts protein C to activated protein C. Activated protein C is an anticoagulant that inactivates coagulation factors Va and VIIIa. Human and rat Thrombomodulin show 67% and 86% amino acid sequence identity, respectively, to mouse Thrombomodulin. In humans, Thrombomodulin plays a role in thrombosis, arteriosclerosis, and cancer. 

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse Thrombomodulin Capture Antibody 1 x 600µl
    10X Mouse Thrombomodulin Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BR 1 x 6ml
    Mouse Thrombomodulin Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
  • Tissue specificity

    Endothelial cells are unique in synthesizing thrombomodulin.
  • Involvement in disease

    Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
    Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similarities

    Contains 1 C-type lectin domain.
    Contains 6 EGF-like domains.
  • Post-translational
    modifications

    N-glycosylated.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • AHUS 6
    • AHUS6
    • BDCA 3
    • BDCA3
    • CD 141
    • CD141
    • CD141 antigen
    • Fetomodulin
    • Thbd
    • THPH12
    • THRM
    • Thrombomodulin
    • TM
    • TRBM_HUMAN
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab209880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Thrombomodulin were measured in duplicates, interpolated from the Thrombomodulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 5%, plasma (EDTA) 5%, and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombomodulin concentration was determined to be 111.2 ng/mL in serum, 88.6 ng/mL in plasma (citrate), 95.4 ng/mL in plasma (EDTA), and 119.6 ng/mL in plasma (heparin).

  • The concentrations of Thrombomodulin were measured in duplicates, interpolated from the Thrombomodulin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 5%, mouse lung supernatant 1:150, and cell culture media 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombomodulin concentration was determined to be 81.9 ng/mL in urine, 805.5 ng/mL in lung supernatant and 25.8 ng/mL in cell culture media. Mouse lung supernatant was cultured for 6 days in cell culture media plus 10% Fetal Bovine Serum and 5 μg/mL LPS.

Protocols

References

This product has been referenced in:

  • Inagawa R  et al. Ultrastructural Alteration of Pulmonary Capillary Endothelial Glycocalyx During Endotoxemia. Chest N/A:N/A (2018). Read more (PubMed: 29555594) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab209880.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up