Overview

  • Product name

    Mouse TIMP1 ELISA Kit
    See all TIMP1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 6.7%
  • Sample type

    Cell culture supernatant, Serum, Plasma, Cell culture extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    6.2 pg/ml
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 111 110% - 113%
    Cell culture extracts 104 90% - 119%
    Cell culture media 107 106% - 108%
    Citrate Plasma 112 109% - 113%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig
  • Product overview

    TIMP1 in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TIMP1 protein in mouse serum, plasma, cell culture supernatant, cell and tissue extracts.


    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.



    Sensitivity:


    Samples diluted in Sample Diluent NS – 6.2 pg/mL
    Samples diluted in 1X Cell Extraction Buffer PTR – 8 pg/mL

  • Notes

    Tissue metalloproteinase inhibitor 1 (TIMP1or Metalloproteinase inhibitor 1) inhibits matrix metalloproteinases (MMP’s) which cause degradation of the extracellular matrix. TIMP1 inhibits MMP’s by forming a non-covalent bond complex at their catalytic zinc cofactor site and irreversibly inactivating them. TIMP1 also promotes cell proliferation and has some anti-apoptotic functions.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab196265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of TIMP1 were measured in duplicate and interpolated from the TIMP1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP1 concentration was determined to be 4,495 pg/mL in Mouse serum and 4,210 pg/mL in Mouse plasma.

  • Background subtracted data values (mean +/- SD, n = 2) are graphed.

  • L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. TIMP1 was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the TIMP1 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 4.1 ng/mL unstimulated, 18.5 ng/mL stimulated. There was no detectable signal in media.

Protocols

References

This product has been referenced in:

  • Tajerian M  et al. The hippocampal extracellular matrix regulates pain and memory after injury. Mol Psychiatry 23:2302-2313 (2018). Read more (PubMed: 30254235) »
See 1 Publication for this product

Customer reviews and Q&As

Extremely easy to use

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Very straightforward kit. I used it in mouse hippocampus lyasates. Takes very little time, is very sensitive, and the instructions are very clear.

Abcam user community

Verified customer

Submitted Mar 21 2017

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