• Product name

    Mouse TIMP2 ELISA Kit
    See all TIMP2 kits
  • Detection method

  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 80 pg/ml
  • Range

    0.082 ng/ml - 60 ng/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.36 82% - 105%
    Serum 92.65 80% - 112%
    Plasma 93.42 81% - 103%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam's TIMP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TIMP2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse TIMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TIMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    260X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse TIMP2 2 vials
    Recombinant Mouse TIMP2 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TIMP2 Microplate (12 x 8 wells) 1 unit
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP2 (also called CSC-21K) is a 24 kDa glycoprotein that is expressed by a variety of cell types. It forms a non covalent, stoichiometric complex with both latent and active MMPs. TIMP2 shows a preference for MMP2. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models. TIMP2 is constitutively produced in low (pg/ml) levels by most cell types in tissue culture. The low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.
  • Cellular localization

  • Alternative names

    • CSC 21K
    • CSC21K
    • D11Bwg1104e
    • DDC8
    • Metalloproteinase inhibitor 2
    • Metalloproteinase inhibitor 2 precursor
    • MGC105282
    • OTTMUSP00000004170
    • OTTMUSP00000004171
    • RP23-394O9.2
    • TIMP 2
    • TIMP metallopeptidase inhibitor 2
    • Tissue inhibitor of metalloproteinase 2
    • Tissue inhibitor of metalloproteinases 2
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab100746

  • Representative Standard Curve using ab100746



This product has been referenced in:

  • Tajerian M  et al. The hippocampal extracellular matrix regulates pain and memory after injury. Mol Psychiatry 23:2302-2313 (2018). Read more (PubMed: 30254235) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

mouse brain tissue homogenate

Excellent Excellent 5/5 (Ease of Use)
Mouse brain tissue homogenates were made in T-PER protein extraction reagent. With a total protein concentration of 6000 ug/ml, TIMP2 could be detected in both 1:2 and 1:4 dilutions. The ELISA assay was performed according to the instructions. We used the overnight incubation approach.

Abcam user community

Verified customer

Submitted Dec 22 2017

Typical ELISA kit

Good Average 3/5 (Ease of Use)
This is a typical Elisa kit that can be done over 1 long day or broken down to two days with an overnight incubation. The instructions are very clear, and the reagents are presented in a way where one can easily use the kit for 2 different experiments.
Good results with mouse hippocampal lysates.

Abcam user community

Verified customer

Submitted Mar 21 2017


We recommend a 2 fold dilution for plasma samples for use with all three of the mouse ELISA kits.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up