Description

Specifications

Our Abpromise guarantee covers the use of ab93304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • Form

    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names

    • ARMD10
    • CD284
    • CD284 antigen
    • Homolog of Drosophila toll
    • hToll
    • TLR 4
    • TLR4
    • TLR4_HUMAN
    • TOLL
    • Toll like receptor 4
    • Toll-like receptor 4
    see all
  • Function

    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • Tissue specificity

    Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • Involvement in disease

    Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Domain

    The TIR domain mediates interaction with NOX4.
  • Post-translational
    modifications

    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • Cellular localization

    Membrane.
  • Information by UniProt

References

ab93304 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.


ab112362 is a recombinant fragment of residues 214-291of human TLR4 protein. This protein has been tagged with a proprietary tag, xxxxxx (which adds ˜26 kDa).


ab93304 is a peptide derived from residues within the region 400-500 of mouse TLR4. The peptide is only 18 amino acid residues long.This peptide is intended to be used as a blocking peptide to anti-TLR4 antibody ab83444.


Unfortunately these are the only two peptides that we have of this protein. We have several primary antibodies against this target which can be viewed from the following link:

https://www.abcam.com/index.html?
pageconfig=searchresults&search=TLR4&pt=1&sk=targ&sv=TLR4&sn=TLR4&l=1&fViewMore=1


I hope this information has been of help. If you require any further information please do not hesitate to ask.

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Answer

Thank you for contacting us. I apologize for the delay. The catalogue number of blocking peptide is ab116167. The price is £110 for 100 ug size. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. The positive controls for ab95023 are cell lysates of A-431, U2OS, U251 MG, Hela cell etc. Please click the following to see the positive control tissue lysates; http://www.proteinatlas.org/ENSG00000168685 We unfortunately cannot offer blocking peptide for this antibody; the peptide is not commercially available. We can certainly offer a blocking peptide for ab103404; let me know if you are interested in buying this we will then publish this product as special request. TLR4 antibody:- We can recommend ab83444; https://www.abcam.com/tlr4-antibody-ab83444.html The blocking peptide for this is ab93304; https://www.abcam.com/TLR4-peptide-ab93304.html The positive control lysates would be Human, Mouse and Rat small intestine; NIH3T3; EL4 etc. CD45 antibody and peptide would be:- ab10559; https://www.abcam.com/CD45-antibody-ab10559.html and ab17550; https://www.abcam.com/CD45-peptide-ab17550.html The positive control will be Jurkat, KM-H2, or human tonsil tissue lysates. Please also check human tissue Atlas for more information about positive control. Polyclonal antibodies can be used for protein quantifications also however because monoclonal antibodies binds more specifically to particular proteins so monoclonal antibodies are recommended more than polyclonal antibodies. The choice antibodies are depends on the type of application used e.g. in sandwich ELISA monoclonal is best. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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