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    mouse-tnf-alpha-antibody-pair-bsa-and-azide-free-ab241672.pdf

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Immunology Innate Immunity Cytokines TNF Superfamily
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RecombinantRabMAb

Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)

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Sandwich ELISA - Recombinant mouse TNF alpha protein standard curve
  • Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
  • Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
  • Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
  • Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
  • Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
  • Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse TNF alpha Antibody Pair - BSA and Azide free
    See all TNF alpha kits
  • Assay type

    ELISA set
  • Range

    7.8 pg/ml - 500 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Antibody Pair can be used to quantify Mouse TNF alpha. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab208348), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Mouse TNF alpha Capture Antibody (unconjugated) 1 x 100µg
    Mouse TNF alpha Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Growth factors
    • TNF
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Types of disease
    • Metabolic disorders
    • Neuroscience
    • Development
    • Neuroscience
    • Diseases
    • Neuroscience
    • Processes
  • Function

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in disease

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P01375 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • APC1
    • APC1 protein
    • Cachectin
    • DIF
    • Differentiation inducing factor
    • Macrophage cytotoxic factor
    • Tnf
    • TNF superfamily member 2
    • TNF superfamily, member 2
    • TNF, macrophage derived
    • TNF, monocyte derived
    • TNF-a
    • TNF-alpha
    • TNFA
    • TNFA_HUMAN
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor (TNF superfamily member 2)
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    • Tumor Necrosis Factor, Membrane Form
    • Tumor necrosis factor, soluble form
    see all
  • Database links

    • Entrez Gene: 21926 Mouse
    • SwissProt: P06804 Mouse
    • Unigene: 1293 Mouse

    Associated products

    • Alternative Versions

      • Mouse TNF alpha ELISA Kit (ab208348)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab241672 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA
    Use at an assay dependent concentration.
    Notes
    Sandwich ELISA
    Use at an assay dependent concentration.

    Images

    • Sandwich ELISA - Recombinant mouse TNF alpha protein standard curve
      Sandwich ELISA - Recombinant mouse TNF alpha protein standard curve

      Background subtracted standard curve using Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672) and Recombinant mouse TNF alpha protein (Active) (ab259411) in sandwich ELISA.
      The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.

    • Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
      Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
      Representative standard curve from corresponding SimpleStep ELISA® Kit (ab208348), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
    • Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
      Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
      Representative standard curve from corresponding SimpleStep ELISA® Kit (ab208348), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
    • Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
      Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
      Representative standard curve from corresponding SimpleStep ELISA® Kit (ab208348), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
    • Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
      Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
      Representative standard curve from corresponding SimpleStep ELISA® Kit (ab208348), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
    • Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
      Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
      Representative standard curve from corresponding SimpleStep ELISA® Kit (ab208348), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
    • Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
      Sandwich ELISA - Mouse TNF alpha Antibody Pair - BSA and Azide free (ab241672)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab241672? Please let us know so that we can cite the reference in this datasheet.

    ab241672 has not yet been referenced specifically in any publications.

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