• Product name
    Mouse TNF alpha ELISA Kit
    See all TNF alpha kits
  • Detection method
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 60 pg/ml
  • Range
    93.75 pg/ml - 6000 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 92.46 84% - 104%
    Plasma 93.82 83% - 102%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s TNF alpha Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF alpha in plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    We have not been able to detect endogenous Mouse TNF alpha in normal serum with ab100747, only in serum spiked with Mouse TNF alpha.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse TNF alpha (lyophilized) 2 vials
    Recombinant Mouse TNF alpha Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
    TNF alpha Microplate (12 x 8 wells) 1 unit
  • Research areas
  • Function
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in disease
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • APC1 protein
    • Cachectin
    • DIF
    • soluble form
    • Tnf
    • TNF a
    • TNF macrophage derived
    • TNF monocyte derived
    • TNF superfamily member 2
    • TNF-a
    • TNF-alpha
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • TNF? was measured in cell culture medium from Raw control cells, or cells stimulated with PMA (10 ng x mL-1; 24 h) and LPS (1 ug x mL-1; 6 h). Results are shown with background signal subtracted (duplicates +/- SD).

  • Representative Standard Curve using ab100747

  • Representative Standard Curve using ab100747



This product has been referenced in:
  • Russo L  et al. Liver-specific rescuing of CEACAM1 reverses endothelial and cardiovascular abnormalities in male mice with null deletion of Ceacam1 gene. Mol Metab 9:98-113 (2018). Read more (PubMed: 29396368) »
  • Tian D  et al. High fat diet sensitizes fibromyalgia-like pain behaviors in mice via tumor necrosis factor alpha. PLoS One 13:e0190861 (2018). Read more (PubMed: 29444083) »

See all 17 Publications for this product

Customer reviews and Q&As

The ELISA assays are compatible with RIPA buffer. However, you should prepare your standards and positive controls in the same buffer.

The Bradford assay is compatible with RIPA buffer if the samples are diluted at least 10-fold with distill...

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Thank you for contacting us.

Although this kit has not been tested on lysates, it will likely work well using at least a 5 fold dilution of Assay Diluent B.

I hope this information is helpful to you. Please do not hesitate t...

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Thank you for contacting us.

Unfortunately, this kit’s cross reactivity with human has not been determined. If it helps, it looks like the homology of the extracellular domain between human and mouse is only around 70-77% which is not si...

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Thank you for your enquiry which has been forwarded to the scientific support team.

I can confirm that these three ELISA kits ab108870 ab100747 and ab100697 have been tested on mouse plasma and serum samples. (We would not recommend to test ...

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Thank you very much for your email and for sending your absorbance values.

I've reviewed this data with our lab, and we do agree that these standard absorbances are weaker than expected. Did yourun any samples as well?This would help narrow d...

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Thank you very much for your call today and for letting us know about the trouble with this new kit.

As we discussed, I'm sending a free of charge replacement kit on the order ***, which I expect to arrive Tuesday or Wednesday of next week. P...

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Abreview for TNF alpha Mouse ELISA Kit

Good Good 4/5 (Ease of Use)

Sample: Mouse Cell (Homogenised lung tissue)
Specification: Homogenised lung tissue
Amount of Sample: 100 µg
Type of Kit Used: ELISA
Positive control: Virally infected lung tissue.
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Overall, a good kit. However, we struggled to detect TNFa at the highest concentration (6000pg/ml) due to too much signal when analysing at 450nm.

Tissue samples (5mg/ml) can be diluted between 1/5-1/100 and attain useful results.

Abcam user community

Verified customer

Submitted Aug 17 2012

Thank you for contacting us.

The following ELISA kits for mouse ICAM, VCAM, IL-6 and TNF-alpha are suitable for mouse samples:

ab100688 (https://www.abcam.com/ICAM1-Mouse-ELISA-Kit-1-x-96-Well-Plate-ab100688.html)
ab100713 (ht...

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Thank you for getting back to me and for sending us your data. I will forward this data and your feedback onto the lab.

Your credit note ID is ***.

I again apologize that thiskit did not perform as stated on the datasheet. As you ...

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Thank you for contacting me about this, and I am sorry to hear that the results were not as expected. I believe that the values given in my previous email were for normal plasma samples, but I will email the lab to check on that.

Could you ju...

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1-10 of 11 Abreviews or Q&A


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