For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
I have finally performed the TNFa assay. Unfortunately, it was a complete waste of time and samples. You said in your last message that the lab measured concentrations between non-detectable, and 2000pg/ml. Were the high samples from treated animals? Is this kit only useful for measuring extremely high, artificially induced levels ofTNF alpha??
My standard curve absorbance range was from 0.3 to 2.5. The samples (plasma diluted 1:2) gave readings in the 0.1 to 0.2 range, resulting in negative values. I presume that the levels of TNF alpha in our samples is not detectable with this kit, but shouldn't the absorbance values fall above the 0 standard???
Asked on Jan 18 2012
Thank you for contacting me about this, and I am sorry to hear that the results were not as expected. I believe that the values given in my previous email were for normal plasma samples, but I will email the lab to check on that.
Could you just send a brief overview of how the plasma samples were prepared? Were the standards diluted in the assay diluent A as well? Could you send a file of your data, as we might be able to suggest some steps to optimize the results?
If the samples and standards were diluted in diluent A, and the sample values are lower than the standards, then there may be some matrix effect from the sample and youcan try a couple of different dilutions to see if this effect can be minimized.
I understand that your samples are limited, and though it may be possible to optimize these results, we do guarantee the kit to work and Ican certainlyarrange a replacement, credit, or refund if you prefer.
Please let me know if you have any questions or if there is anything else that we can do for you, and I will be happy to help.
Answered on Jan 18 2012