For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Thank you for your quick response. I have attached 2 files; one is the complete data from the spec (long) and the other (short) is the same data, but I eliminated some of the points on the std curve to get a better correlation. You will notice that I added one extra point between the zero and the first recommended dilution. For each sample, I did 2 wells at a 1:2 dilution, and 1 well of 100 ul straight plasma. So for the first sample, unknown #1 is duplicates of the diluted sample and unknown #2 is the undiluted sample.
The plasma was collected from mice being fed a diet that causes liver inflammation. Blood was drawn (plus EDTA) and centrifuged to separate the plasma from cells. The samples were frozen at -80 before analysis. Yes, both samples and standards were diluted in Diluent A.
I suspect that detectable levels of TNFa are only present in samples from animals treated with LPS or other extremely inflammatory treatments, and that TNFa is not detectable in our samples with this kit. We would appreciate a refund in that case.
Asked on Jan 18 2012
Thank you for getting back to me and for sending us your data. I will forward this data and your feedback onto the lab.
Your credit note ID is ***.
I again apologize that thiskit did not perform as stated on the datasheet. As you have paid by credit card, the purchase price (including shipping and taxes) will be refunded to your card as soon as our accounting department approves.
If you have any questions about this refund, our accounting department can be contacted by email at email@example.com or by telephone at 888-772-2226. Please refer to the credit note ID in any correspondence with our accounting department.
Please let me know if there is anything else that we can do for you in the future, and I wish you the best of the luck with your research.
Answered on Jan 18 2012