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I have a customer who is interested in measuring TNF levels in mouse samples. Their assay is different in that they first treat the mouse samples with human TNF.
They have previously used ELISA kits from other companies, however these kits have also detected the human TNF in the samples.
Does Abcam have a suitable ELISA kit that would detect mouse TNF and not human TNF? Would ab100747 be suitable?
I look forward to your response.
Asked on Feb 21 2013
Thank you for contacting us.
Unfortunately, this kit’s cross reactivity with human has not been determined. If it helps, it looks like the homology of the extracellular domain between human and mouse is only around 70-77% which is not significantly high and both antibodies used are monoclonal so the kit is quite specific for mouse.
The only simple way to know for sure would be to spike in only human TNF alpha and see if the assay detects it. I wish I could give a more definite answer on this one.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Feb 21 2013