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Inquiry: TNF alpha Mouse ELISA Kit (ab100747) Please advise on usage of Assay Diluent A or Assay Diluent B for mouse tissue lysates to detect level of TNF alpha using TNF alpha Mouse ELISA Kit (ab100747).
Asked on Aug 12 2013
Thank you for contacting us.
Although this kit has not been tested on lysates, it will likely work well using at least a 5 fold dilution of Assay Diluent B.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Heather AllenAbcam Scientific Support
Answered on Aug 12 2013