Overview

  • Product name
    Mouse TNF alpha ELISA Kit
    See all TNF alpha kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    30 pg/ml
  • Range
    0.031 ng/ml - 2 ng/ml
  • Recovery

    97 %

  • Assay time
    5h 0m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s TNF alpha Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TNF alpha levels in plasma, serum and cell culture supernatants.

    A TNF alpha specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TNF alpha specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TNF alpha captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Mouse TNF alpha Antibody 1 x 140µl
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
    TNF alpha Microplate (12 x 8 well strips) 1 unit
    TNF alpha Standard 1 vial
  • Research areas
  • Function
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in disease
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • APC1 protein
    • Cachectin
    • DIF
    • soluble form
    • Tnf
    • TNF a
    • TNF macrophage derived
    • TNF monocyte derived
    • TNF superfamily member 2
    • TNF-a
    • TNF-alpha
    • TNFA_HUMAN
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab108910 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108910

    Protocols

    References

    This product has been referenced in:
    • Wang H  et al. AMPKa2 deficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction. Free Radic Biol Med 121:202-214 (2018). Read more (PubMed: 29753072) »
    • Zheng J  et al. Maternal Low-Protein Diet Modulates Glucose Metabolism and Hepatic MicroRNAs Expression in the Early Life of Offspring †. Nutrients 9:N/A (2017). ELISA ; Mouse . Read more (PubMed: 28264458) »

    See all 2 Publications for this product

    Publishing research using ab108910? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    3 reviews or Q&A

    10
    Votes: Highest First

    We used RIPA lysis buffer and wondered whether it will have an effect on results. The RIPA buffer includes 150mM NaCl, 0.1% Triton X-100, 0.1% SDS, 0.5% sodium deoxycholate, and 50mM Tris-HCl. Another question that we have is that we used the Bradford ...

    Read More

    The ELISA assays are compatible with RIPA buffer. However, you should prepare your standards and positive controls in the same buffer.

    The Bradford assay is compatible with RIPA buffer if the samples are diluted at least 10-fold with distill...

    Read More

    Hello, I am using ELISA kit number ab108910. I have an older plate reader on an older computer, and I cannot change the program settings for the reader. This ELISA kit comes with a white tray with clear strips of tubes, but I cannot change the setting ...

    Read More

    Thank you for contacting us.

    Our kit is color metric not chemoluminescent or fluorescent format. Only those format come with black frame. I don’t think the frame is an issue for the reader.


    I hope this information is helpfu...

    Read More

    Hello, Do you have a protocol for culturing cells to use the supernant for ELISAs? I am using your kits (ab108910 and ab108870) to detect TNFa and IL-10 in the supernatant from mouse macrophages, but I am having  (excel file attached). Any advice would...

    Read More

    Thank you for contacting us.

    I am very sorry to hear that you have been having troubles when using this product. In order that I might more fully understand the problem could you please tell me what is sample dilution factor was that you u...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up