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Hello, Do you have a protocol for culturing cells to use the supernant for ELISAs? I am using your kits (ab108910 and ab108870) to detect TNFa and IL-10 in the supernatant from mouse macrophages, but I am having (excel file attached). Any advice would be appreciated.
Asked on Nov 29 2012
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I am very sorry to hear that you have been having troubles when using this product. In order that I might more fully understand the problem could you please tell me what is sample dilution factor was that you used? Sample concentration, both too high or too low can often, can lead to problems such as we see here. Next, how where your cell culture media samples collected and prepared? Were all reagents at room temperature before use?
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Answered on Nov 29 2012