Mouse TNF alpha Matched Antibody Pair Kit (ab212073)

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Overview

  • Product name

    Mouse TNF alpha Matched Antibody Pair Kit
    See all TNF alpha kits

  • Detection method

    Colorimetric

  • Assay type

    ELISA set

  • Range

    7.8 pg/ml - 500 pg/ml

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse TNF alpha. Both capture and detector antibodies are rabbit monoclonal antibodies.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification. 


    Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. 


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Mouse TNF alpha Capture Antibody 1 x 100µg 1 x 20µg
    Mouse TNF alpha Detector Antibody 1 x 25µg 1 x 5µg
    Mouse TNF alpha Lyophilized Protein 1 vial 1 vial

  • Research areas

  • Function

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

  • Involvement in disease

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

  • Sequence similarities

    Belongs to the tumor necrosis factor family.

  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Alternative names

    • APC1
    • APC1 protein
    • Cachectin
    • DIF
    • Differentiation inducing factor
    • Macrophage cytotoxic factor
    • Tnf
    • TNF superfamily member 2
    • TNF superfamily, member 2
    • TNF, macrophage derived
    • TNF, monocyte derived
    • TNF-a
    • TNF-alpha
    • TNFA
    • TNFA_HUMAN
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor (TNF superfamily member 2)
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    • Tumor Necrosis Factor, Membrane Form
    • Tumor necrosis factor, soluble form
    see all

  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab212073 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA Use at an assay dependent concentration.

    Images

    • Mouse TNFa standard curve
      Mouse TNFa standard curve

      Standard calibration curve. Background subtracted values are graphed.

    Protocols

    References

    This product has been referenced in:

    • Wu Y  et al. Microglia and amyloid precursor protein coordinate control of transient Candida cerebritis with memory deficits. Nat Commun 10:58 (2019). Read more (PubMed: 30610193) »
    See 1 Publication for this product

    Publishing research using ab212073? Please let us know so that we can cite the reference in this datasheet.

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