Mouse TNFRSF14 ELISA Kit (HVEM) (ab213892)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TNFRSF14 ELISA Kit (HVEM)
See all TNFRSF14/HVEM kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 29pg/ml 1.59 = 5.5% 2 16 218pg/ml 14.38 = 6.6% 3 16 439pg/ml 26.77 = 6.1% Inter-assay Sample n Mean SD CV% 1 24 28pg/ml 1.56 = 5.6% 2 24 197pg/ml 16.15 = 8.2% 3 24 475pg/ml 31.82 = 6.7% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 1 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse TNFRSF14 Enzyme-Linked Immunosorbent Assay (ELISA) kit (HVEM) (ab213892) is designed for the quantitative detection of Mouse TNFRSF14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for TNFRSF14/HVEM has been precoated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: Q29-V207) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF14/HVEM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TNFRSF14/HVEM amount of sample captured in plate.
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Notes
Tumor necrosis factor receptor superfamily member 14 (TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which form a bidirectional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Adhesive Plate Seal 4 units 4 units Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Mouse TNFRSF14 coated Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti- Mouse TNFRSF14 antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Mouse TNFRSF14 standard 2 vials 2 vials Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
Research areas
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Function
Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells. -
Tissue specificity
Widely expressed, with the highest expression in lung, spleen and thymus. -
Sequence similarities
Contains 3 TNFR-Cys repeats. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- HVEML
- ATAR
- CD270
see all -
Database links
- Entrez Gene: 230979 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab213892 has not yet been referenced specifically in any publications.