Overview

  • Product name

    Mouse TNFRSF17 ELISA Kit (BCMA)
    See all BCMA kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 126pg/ml 5.67 4.5%
    2 16 755pg/ml 24.16 3.2%
    3 16 1323pg/ml 70.12 5.3%
    Inter-assay
    Sample n Mean SD CV%
    1 24 240pg/ml 13.92 5.8%
    2 24 875pg/ml 40.25 4.6%
    3 24 1434pg/ml 88.91 6.2%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    31.2 pg/ml - 2000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Mouse TNFRSF17 Enzyme-Linked Immunosorbent Assay (ELISA) kit (BCMA) (ab213893) is designed for the quantitative detection of Mouse TNFRSF17 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for TNFRSF17/BCMA has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: M1-T49) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF17/BCMA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TNFRSF17/BCMA amount of sample captured in plate. 

  • Notes

    TNFRSF17 (Tumor necrosis factor receptor superfamily member 17), also called BCMA, is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily and is mapped to 16p13.13. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Mouse TNFRSF17 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Mouse TNFRSF17 antibody 1 x 130µl
    Lyophilized recombinant Mouse TNFRSF17 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.
  • Tissue specificity

    Expressed in mature B-cells, but not in T-cells or monocytes.
  • Involvement in disease

    Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.
  • Sequence similarities

    Contains 1 TNFR-Cys repeat.
  • Cellular localization

    Cell membrane. Endomembrane system. Perinuclear Golgi-like structures.
  • Information by UniProt
  • Alternative names

    • B cell maturation antigen
    • B cell maturation factor
    • B cell maturation protein
    • B-cell maturation protein
    • BCM
    • BCMA
    • CD269
    • CD269 antigen
    • TNFRSF17
    • TNR17_HUMAN
    • Tumor necrosis factor receptor superfamily member 17
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse TNFRSF17 ELISA Kit (BCMA) (ab213893) Standard Curve.

Protocols

References

ab213893 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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