Mouse TNFRSF17 ELISA Kit (BCMA) (ab213893)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse TNFRSF17 ELISA Kit (BCMA)
See all BCMA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 126pg/ml 5.67 4.5% 2 16 755pg/ml 24.16 3.2% 3 16 1323pg/ml 70.12 5.3% Inter-assay Sample n Mean SD CV% 1 24 240pg/ml 13.92 5.8% 2 24 875pg/ml 40.25 4.6% 3 24 1434pg/ml 88.91 6.2% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
31.2 pg/ml - 2000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse TNFRSF17 Enzyme-Linked Immunosorbent Assay (ELISA) kit (BCMA) (ab213893) is designed for the quantitative detection of Mouse TNFRSF17 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for TNFRSF17/BCMA has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: M1-T49) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF17/BCMA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TNFRSF17/BCMA amount of sample captured in plate.
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Notes
TNFRSF17 (Tumor necrosis factor receptor superfamily member 17), also called BCMA, is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily and is mapped to 16p13.13. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Mouse TNFRSF17 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti- Mouse TNFRSF17 antibody 1 x 130µl Lyophilized recombinant Mouse TNFRSF17 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK. -
Tissue specificity
Expressed in mature B-cells, but not in T-cells or monocytes. -
Involvement in disease
Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2. -
Sequence similarities
Contains 1 TNFR-Cys repeat. -
Cellular localization
Cell membrane. Endomembrane system. Perinuclear Golgi-like structures. - Information by UniProt
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Alternative names
- B cell maturation antigen
- B cell maturation factor
- B cell maturation protein
see all -
Database links
- Entrez Gene: 21935 Mouse
- SwissProt: O88472 Mouse
- Unigene: 12935 Mouse
Applications
Our Abpromise guarantee covers the use of ab213893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Mouse TNFRSF17 ELISA Kit (BCMA) (ab213893) Standard Curve.Mouse TNFRSF17 ELISA Kit (BCMA) (ab213893) Standard Curve.
Protocols
References
ab213893 has not yet been referenced specifically in any publications.
