Overview

  • Product name
    Mouse TNFRSF4 ELISA Kit (OX40)
    See all CD134 / OX40L receptor kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    1 16 224pg/ml 7.62 3.4%
    2 16 732pg/ml 32.94 4.5%
    3 16 1463pg/ml 83.4 5.7%
    Inter-assay
    Sample n Mean SD CV%
    1 24 364pg/ml 17.5 4.8%
    2 24 885pg/ml 49.56 5.6%
    3 24 1376pg/ml 95 6.9%
  • Sample type
    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    31.2 pg/ml - 2000 pg/ml
  • Assay time
    3h 30m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    The Mouse TNFRSF4 Enzyme-Linked Immunosorbent Assay (ELISA) kit (OX40) (ab213894) is designed for the quantitative detection of Mouse TNFRSF4 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for TNFRSF4/OX40 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: V20-P211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF4/OX40 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TNFRSF4/OX40 amount of sample captured in plate. 

  • Notes

    Tumor necrosis factor receptor superfamily, member 4, also known as ACT35 or CD134 is a cell surface glycoprotein that was discovered through the production of a monoclonal antibody raised against the HUT-102 cell line. It belongs to the tumor necrosis factor receptor superfamily. CD134 was mapped to 1p36 by fluorescence in situ hybridization. CD134 is the primary receptor for feline immunodeficiency virus. CD134 expression can promote viral binding and renders cells permissive for viral entry, productive infection, and syncytium formation. Stimulating the receptor can improve the response to a powerful virus vector and may be useful in vaccine development.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab213894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse TNFRSF4 ELISA Kit (OX40) (ab213894) Standard Curve.

Protocols

References

ab213894 has not yet been referenced specifically in any publications.

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