Overview

  • Product name

    Mouse TNFRSF7 ELISA Kit (CD27)
    See all CD27 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 533pg/ml 22.4 4.2%
    2 16 1626pg/ml 56.91 3.5%
    3 16 2563pg/ml 138.4 5.4%
    Inter-assay
    Sample n Mean SD CV%
    1 24 658pg/ml 39.5 6%
    2 24 1856pg/ml 89.1 4.8%
    3 24 2731pg/ml 180.3 6.6%
  • Sample type

    Cell culture supernatant, Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Mouse TNFRSF7 Enzyme-Linked Immunosorbent Assay (ELISA) kit (CD27) (ab213895) is designed for the quantitative detection of Mouse TNFRSF7 in cell culture supernatants and serum.


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for TNFRSF7/CD27 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: T21-R182) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF7/CD27 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TNFRSF7/CD27 amount of sample captured in plate. 

  • Notes

    CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab213895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse TNFRSF7 ELISA Kit (CD27) (ab213895) Standard Curve.

Protocols

References

ab213895 has not yet been referenced specifically in any publications.

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