Mouse TNFRSF7 ELISA Kit (CD27) (ab213895)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TNFRSF7 ELISA Kit (CD27)
See all CD27 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 533pg/ml 22.4 4.2% 2 16 1626pg/ml 56.91 3.5% 3 16 2563pg/ml 138.4 5.4% Inter-assay Sample n Mean SD CV% 1 24 658pg/ml 39.5 6% 2 24 1856pg/ml 89.1 4.8% 3 24 2731pg/ml 180.3 6.6% -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse TNFRSF7 Enzyme-Linked Immunosorbent Assay (ELISA) kit (CD27) (ab213895) is designed for the quantitative detection of Mouse TNFRSF7 in cell culture supernatants and serum.
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for TNFRSF7/CD27 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: T21-R182) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF7/CD27 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TNFRSF7/CD27 amount of sample captured in plate.
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Notes
CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Mouse TNFRSF7 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Mouse TNFRSF7 antibody 1 x 100µl Lyophilized recombinant Mouse TNFRSF7 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. -
Tissue specificity
Found in most T-lymphocytes. -
Sequence similarities
Contains 3 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD 27
- CD27
- CD27 antigen
see all -
Database links
- Entrez Gene: 21940 Mouse
- SwissProt: P41272 Mouse
- Unigene: 367714 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab213895 has not yet been referenced specifically in any publications.