Overview

  • Product name

    Mouse TrkB ELISA Kit
    See all TrkB kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Cell Lysate, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 5 pg/ml
  • Range

    93.8 pg/ml - 6000 pg/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s mouse TrkB in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Mouse TrkB in cell culture supernatants, cell lysates and tissue homogenates.


    A TrkB specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for TrkB is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse TrkB amount of sample captured in plate.


    Get higher sensitivity in only 90 minutes with Mouse TrkB ELISA Kit (ab203368) from our SimpleStep ELISA® range.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab119610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab119610

Protocols

References

ab119610 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

Unfortunately, this kit cannot be used with rat samples.

We have 2 TrkB antibodies (ab51190 and ab43079) that have been tested in indirect ELISA and rat samples, and might work together in a sandwich ELISA. However, we have not tried this yet. But you could use our testing discount program if you wanted to them out.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

Please let me know if you would be interested in this offer.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your enquiry.

I'll briefly describe the way our product discount testing program works.

1. Contact Abcam Scientific Support to discuss your novel use of the product
2. If we approve of your testing the product, you will receive an email with a discount code associated with your specific testing conditions and an expiration date for the code
3. You would then purchase the kit as you normally would, test it in the manner we discussed and lastly submit an onlineAbreview relating your experience.
4. Once we receive your Abreview, your code is activated
5. You can then order another ELISA kit,providing the discountcode at time of order. Once customer service confirms submission of the Abreview, they will remove 100% of the purchase price from the cost of this item

I hope this is clear. Please feel free to contact me with any questions.

Read More

Question
Answer

DISCOUNT CODE: ***
Expiration date: June 15th, 2012

I am very pleased to hear you would like to accept our offer and test ab119609 in mouse. This code will give you 1 free ELISA kit before the expiration date. To redeem this offer, please submit an Abreview for ab119609 and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your patience.

The lab informed me thatRIPA buffer is compatible with the kit and can be usedwithtissue.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up