Overview

  • Product name

    Mouse TrkB ELISA Kit
    See all TrkB kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 3.2%
  • Sample type

    Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    33.3 pg/ml
  • Range

    78 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Mouse brain extract 106 103% - 110%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s TrkB in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TrkB protein in mouse cell and tissue extracts.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    TrkB is a tyrosine kinase receptor involved in the development of the peripheral and central nervous system. TrkB plays a role in synapse plasticity and formation, and neuron survival, proliferation and differentiation. Ligand (including BDNF and NTF4) interactions induces homodimerization, autophosphorylation and activation of TrkB. Activity of TrkB is also affected by the formation of inactive heterodimers with noncatalytic isoforms.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab203368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated values of TrkB are plotted based on an extract load of 50 µg/mL. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Interpolated values from the mouse protein standard are graphed.

Protocols

References

ab203368 has not yet been referenced specifically in any publications.

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