Mouse TSLP ELISA Kit (ab155461)
- Datasheet
- References
- Protocols
Overview
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Product nameMouse TSLP ELISA Kit
See all TSLP kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% overall < 10% Inter-assay Sample n Mean SD CV% overall < 12% -
Sample typeCell culture supernatant, Serum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity< 1 pg/ml
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Range0.82 pg/ml - 200 pg/ml
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Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 126.4 98% - 125% Serum 117.5 105% - 129% Plasma 98.55 86% - 122% -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Mouse
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Product overview
Abcam’s TSLP Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse TSLP in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse TSLP coated on a 96- well plate. Standards and samples are pipetted into the wells and TSLP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TSLP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TSLP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-mouse TSLP 2 vials Recombinant Mouse TSLP Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml TSLP Microplate (12 x 8 wells) 1 unit -
Research areas
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RelevanceThe TSLP gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. This mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, chronic obstructive pulmonary disease and allergic inflammation. The protein is therefore considered a potential therapeutic target for the treatment of these diseases. Alternative splicing of this gene results in multiple transcript variants.
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Cellular localizationSecreted
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Alternative names
- Thymic stromal lymphopoietin
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Database links
- Entrez Gene: 53603 Mouse
- SwissProt: Q9JIE6 Mouse
- Unigene: 143716 Mouse
Applications
Our Abpromise guarantee covers the use of ab155461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative standard curve using ab155461 - Assay Diluent B.
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Typical Standard CurveRepresentative standard curve using ab155461 - Assay Diluent A.
Datasheets and documents
Protocols
References
ab155461 has not yet been referenced specifically in any publications.
