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    mouse-tslp-elisa-kit-ab155461.pdf

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Immunology Innate Immunity Cytokines Other
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Mouse TSLP ELISA Kit (ab155461)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve
  • Typical Standard Curve

Key features and details

  • Sensitivity: 1 pg/ml
  • Range: 0.82 pg/ml - 200 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse TSLP ELISA Kit
    See all TSLP kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 1 pg/ml
  • Range

    0.82 pg/ml - 200 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 126.4 98% - 125%
    Serum 117.5 105% - 129%
    Plasma 98.55 86% - 122%
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s TSLP Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse TSLP in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse TSLP coated on a 96- well plate. Standards and samples are pipetted into the wells and TSLP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TSLP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TSLP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-mouse TSLP 2 vials
    Recombinant Mouse TSLP Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
    TSLP Microplate (12 x 8 wells) 1 unit
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Other
    • Immunology
    • Innate Immunity
    • Mast Cells
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
  • Relevance

    The TSLP gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. This mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, chronic obstructive pulmonary disease and allergic inflammation. The protein is therefore considered a potential therapeutic target for the treatment of these diseases. Alternative splicing of this gene results in multiple transcript variants.
  • Cellular localization

    Secreted
  • Alternative names

    • Thymic stromal lymphopoietin
  • Database links

    • Entrez Gene: 53603 Mouse
    • SwissProt: Q9JIE6 Mouse
    • Unigene: 143716 Mouse

    Associated products

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab155461 - Assay Diluent B.

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab155461 - Assay Diluent A.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab155461? Please let us know so that we can cite the reference in this datasheet.

      ab155461 has not yet been referenced specifically in any publications.

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