Overview

  • Product name

    Mouse Tweak ELISA Kit
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 0.3 pg/ml
  • Range

    0.27 pg/ml - 200 pg/ml
  • Recovery

    77 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75.69 68% - 83%
    Serum 79.66 71% - 87%
    Plasma 77.72 70% - 89%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s Tweak Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Tweak in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Tweak coated on a 96-well plate. Standards and samples are pipetted into the wells and Tweak present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Tweak antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Tweak bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab113320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab113320.

Protocols

References

ab113320 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Suite à mon dernier email, j’ai eu un supplément d’informations du laboratoire. Voir les pièces jointes.

Notez aussi que le standard ne doit pas être vortexer pour être mélanger mais plutôt solution C devrait être brièvement centrifugé avant l'ouverture puis une fois le diluant ajouté, la solution doit être mélangé en pipetant doucement la solution.

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Answer

Je suis en contact avec le laboratoire pour résoudre ce cas.

En attendant, j’aimerais vous demander les questions suivantes :

Combien de fois avez vous effectué ces manips ?
Comment avez-vous stocké ces kits ?
Avez-vous brièvement vortexer « item c » avant utilisation ? Est-ce que la poudre a été bien dissoute ?
Combien de fois avez-vous lavé la plaque ?
Avez-vous ouvert la plaque juste avant utilisation ?
Est-ce que les ODs ont été déterminés juste après que le « stop solution » a été ajouté ?

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Answer

Thank you for your patience.

I am sorry this item G of ab113320 did not containthe volumeas stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx for 1 vial item G. As soon as we receive the vial it will ship to you - most likely early next week. The estimated delivery date is set for xxx.

The vialwill be the new 200ul of 200X concentration, thus pleasedilute the component accordingly. Please alsospin the vialprior to opening to ensure complete recovery of the liquid at the bottomof the vial.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Answer

Thank you for your patience.

I will be arranging for sending you 1 vial of item G (200ul, 200x). The process will be taking about a week and I expect that you will receive it next week.

The online datasheet states incorrectly 240x, but the online protocol is correct in stating 200x. I'll have the datasheet information updated as quickly as possible. The volume of 200 ul is correct, though. I apologize about this confusion.

Thank you for your understanding.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting Abcam regarding ab113320.


This ELISA kit has not been tested against monomeric TWEAK/TNFSF12. It is only known to detect mouse TWEAK in its trimeric form. It is important to note that TWEAK is a homotrimer, meaning that all of the three units of the peptide are identical. Therefore, it is likely that the antibodies will recognize the monomers of TWEAK, but it is still unknown.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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Answer

Thank you for contacting us.
I am sorry about the problems you have encountered with our kits.

I have emailed the lab to replace the kit component item G (lot GR650572-2) which you had received empty. I will be in touch with more information in this regard.

Please let me know if I can help you with anything else in the meantime.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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