• Product name
    Mouse Tweak ELISA Kit
    See all Tweak kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.3 pg/ml
  • Range
    0.27 pg/ml - 200 pg/ml
  • Recovery

    77 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75.69 68% - 83%
    Serum 79.66 71% - 87%
    Plasma 77.72 70% - 89%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Tweak Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Tweak in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Tweak coated on a 96-well plate. Standards and samples are pipetted into the wells and Tweak present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Tweak antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Tweak bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform



Our Abpromise guarantee covers the use of ab113320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab113320.



ab113320 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Suite à mon dernier email, j’ai eu un supplément d’informations du laboratoire. Voir les pièces jointes.

Notez aussi que le standard ne doit pas être vortexer pour être mélanger mais plut&oc...

Read More

Je suis en contact avec le laboratoire pour résoudre ce cas.

En attendant, j’aimerais vous demander les questions suivantes :

Combien de fois avez vous effectué ces manips ?
Comment avez-vous stocké c...

Read More

Thank you for your patience.

I am sorry this item G of ab113320 did not containthe volumeas stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx...

Read More

Thank you for your patience.

I will be arranging for sending you 1 vial of item G (200ul, 200x). The process will be taking about a week and I expect that you will receive it next week.

The online datasheet states incorrectly 240x,...

Read More

Thank you for contacting Abcam regarding ab113320.

This ELISA kit has not been tested against monomeric TWEAK/TNFSF12. It is only known to detect mouse TWEAK in its trimeric form. It is important to note that TWEAK is a homotrimer, mean...

Read More

Thank you for contacting us.
I am sorry about the problems you have encountered with our kits.

I have emailed the lab to replace the kit component item G (lot GR650572-2) which you had received empty. I will be in touch with more inform...

Read More


Sign up