Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 0.4 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse TYRO3 ELISA Kit (DTK)
See all TYRO3 kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.4 ng/ml - 100 ng/ml
Sample specific recovery Sample type Average % Range Serum 71.55 67% - 80% Plasma 72.51 68% - 78% Cell culture media 71.99 68% - 76%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Mouse TYRO3 ELISA Kit is designed for the quantitative determination of TYRO3 in cell culture supernatants, plasma and serum samples.
? Note: Mouse TYRO3 concentration is low in normal serum and plasma samples, and may not be detectable in this assay.
This assay employs an antibody specific for mouse TYRO3 coated on a 96-well plate. Standards and samples are pipetted into the wells and TYRO3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TYRO3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TYRO3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- ?, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 ?, IL-1?, IL-2, IL-3, IL3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1?, MIP-1?, MIP-2, MIP-3?, MIP-3?, PF-4, P-Selectin, RANTES, SCF, SDF-1?, TARC, TCA-3, TECK, TIMP-1, TNF-?, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Mouse TYRO3 coated Microplate (12 x 8 wells) 1 unit Assay Diluent A 1 x 30ml Biotinylated Anti-Mouse TYRO3 Detection Antibody 2 vials Mouse TYRO3 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
FunctionMay be involved in cell adhesion processes, particularly in the central nervous system. In case of filovirus infection, seems to function as a cell entry factor.
Tissue specificityAbundant in the brain and lower levels in other tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Cellular localizationCell membrane.
- Information by UniProt
ab267577 has not yet been referenced specifically in any publications.