Overview

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Accession
    • Species
      Mouse
    • Sequence
      RALMKVQVLRESPF
    • Amino acids
      295 to 308

Specifications

Our Abpromise guarantee covers the use of ab4994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • Form
    Liquid
  • Additional notes


    This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and mouse UCP 3, catalog ab3477. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~ 70-fold) for competitive inhibition of antibody-protein binding reactions.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

General Info

  • Alternative names
    • Mitochondrial uncoupling protein 3
    • SLC25A9
    • Solute carrier family 25 member 9
    • UCP 3
    • UCP3
    • UCP3_HUMAN
    • Uncoupling protein 3
    • Uncoupling protein 3 mitochondrial proton carrier
    see all
  • Function
    UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.
  • Tissue specificity
    Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.
  • Involvement in disease
    Defects in UCP3 may be involved in obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similarities
    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt

References

This product has been referenced in:
  • Harmancey R  et al. Chronic Hyperinsulinemia Causes Selective Insulin Resistance and Down-regulates Uncoupling Protein 3 (UCP3) through the Activation of Sterol Regulatory Element-binding Protein (SREBP)-1 Transcription Factor in the Mouse Heart. J Biol Chem 290:30947-61 (2015). Read more (PubMed: 26555260) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thank you for your enquiry. Here is the protocol: BLOCKING WITH IMMUNIZING PEPTIDE (BL) PROTOCOL It is not uncommon to see more than one band in Western blotting when probing with a given antibody or to see more diffuse staining in immunolocalization studies. The question arises which band or staining is specific. The antibody specificity is generally studied by competing with excess antigen (peptide or protein) or immuno-neutralization with the antigens. In principle, a small volume of antibody (e.g. 1-5 µl) is first reacted with excess peptide (5-50 fold over the antibody; e.g. 1 µg antibody reacted with 5-50 µg peptide; exact amounts determined by titration) to neutralize it. The neutralized antibody can no longer bind to another antigen. So the band(s)/staining that is competed by the antigen/peptide is specific. If more than one band disappears by peptide/antigen competition then those bands have the antigenic determinants and could be considered either fragments of the large antigen or multimer. 1. Determine the amount of antibody that is needed for 1 strip (e.g., 2 ml). For example, an antibody has given desired bands at 1:1000 dilution. So you will need 1 µl/ml antibody (2 µl antibody for 2 ml antibody solution). If an antibody was used at 1:5000 dilution then you would only need 0.2 µl/ml (use 2 µl of 1:10 dilution for better accuracy). 2. Take 2 µl antibody (or as needed) in 100 µl saline/PBS. Make 2 tubes. Add antigen/peptide solution (10- 50 µg peptide or antigen added in 10-100 µl). Add same volume of saline/PBS (no peptide/antigen) to the other tube labeled as “no peptide”. Mix gently. 3. Incubate both tubes at 37°C for 1-2 hrs or 2-24 hrs at 4°C. 4. Centrifuge the tubes for 15 min at 4°C in a microfuge (10-15000 rpm) to pellet any immune complexes. Carefully remove the supernatant. If no visible pellet is seen then just leave ~ 5-10 µl at the bottom to avoid disturbing invisible immune complexes. If you do not centrifuge the solution, it may give high background. 5. After centrifugation, make up the volume of supernatant to 2 ml (or what is necessary depending upon the initial antibody taken) with buffer (PBS-Tween with or without BSA or milk or any buffer that was used initially). Use both antibodies (with and without antigen/peptide) for Western blotting or immunolocalization. 6. Observe the bands/staining that disappears. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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