Overview

  • Product name

    Mouse uPA Receptor/U-PAR ELISA Kit
    See all uPA Receptor/U-PAR kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 256pg/ml 13.06 5.1%
    2 16 1862pg/ml 8.38 4.5%
    3 16 3015pg/ml 114.6 3.8%
    Inter-assay
    Sample n Mean SD CV%
    1 24 362pg/ml 23.2 6.4%
    2 24 2165pg/ml 114.7 5.3%
    3 24 3230pg/ml 190.6 5.9%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 5 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Mouse uPA Receptor/U-PAR Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213896) is designed for the quantitative detection of Mouse uPAR in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for uPAR has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: L24-T297) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for uPA Receptor/U-PAR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse uPA Receptor/U-PAR amount of sample captured in plate.

  • Notes

    The urokinase-type plasminogen activator receptor (uPA Receptor/U-PAR) is a key molecule in the regulation of cell surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes. The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosylphosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator. uPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer. PLAUR is located at chromosome 19q13.1-q13.2.1.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Mouse uPA Receptor/U-PAR coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Mouse uPA Receptor/U-PAR antibody 1 x 130µl
    Lyophilized recombinant Mouse uPA Receptor/U-PAR standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.
  • Tissue specificity

    Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.
  • Sequence similarities

    Contains 3 UPAR/Ly6 domains.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names

    • CD 87
    • CD87
    • CD87 antigen
    • MO 3
    • MO3
    • Monocyte activation antigen Mo3
    • Plasminogen activator receptor urokinase
    • Plasminogen activator urokinase receptor
    • PLAUR
    • U PAR
    • u plasminogen activator receptor
    • U-PAR
    • u-plasminogen activator receptor form 2
    • UPA receptor
    • uPAR
    • UPAR_HUMAN
    • Urinary plasminogen activator receptor
    • URKR
    • Urokinase plasminogen activator receptor
    • Urokinase plasminogen activator surface receptor
    • urokinase-type plasminogen activator (uPA) receptor
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse uPAR ELISA Kit (ab213896) Standard Curve.

Protocols

References

ab213896 has not yet been referenced specifically in any publications.

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