Product nameMouse uPA Total ELISA Kit (URK)
See all uPA kits
Intra-assay Sample n Mean SD CV% Known Conc 20 0.06ng/ml 0.005 8.12% Known Conc 20 0.3ng/ml 0.019 6.18% Known Conc 20 3.01ng/ml 0.112 3.73% Inter-assay Sample n Mean SD CV% Known Conc 10 0.09ng/ml 0.008 8.52% Known Conc 10 0.25ng/ml 0.019 7.47% Known Conc 10 2.66ng/ml 0.154 5.79%
Range0.01 ng/ml - 10 ng/ml
Sample specific recovery Sample type Average % Range Spike = 97.7 89.3% - 106%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Does not react with: Sheep
Abcam’s uPA Total (URK) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of total urokinase plasminogen activator antigen in mouse plasma.
Mouse uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-murine uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then bound by the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.
Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-mouse uPA primary antibody 1 vial Anti-rabbit horseradish peroxidase secondary antibody 1 vial Mouse uPA standard 1 vial TMB substrate solution 1 x 10ml uPA Antibody pre-coated 96 well microplate (12 x 8 well strips) 1 x 96 tests
RelevanceUrokinase type plasminogen activator (uPA) is secreted from cells as a precursor which is activated to the two chain form consisting of an A and B chain. The active high MW form is further processed by removal of an amino terminal fragment to an active low MW form (35 kDa). uPA is a serine protease that activates plasminogen toplasmin. High levels of uPA and plasminogen activator inhibitor type 1 (PAI 1) in breast cancer tissue extracts have been associated with rapid disease progression. The malignant phenotype of prostatic tumor cells correlates with the expression of both uPA and its cell membrane receptor (uPAR).
- EC 220.127.116.11
Our Abpromise guarantee covers the use of ab198512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab198512 has not yet been referenced specifically in any publications.