Overview

  • Product name

    Mouse uPA Total ELISA Kit (URK)
    See all uPA kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Known Conc 20 0.06ng/ml 0.005 8.12%
    Known Conc 20 0.3ng/ml 0.019 6.18%
    Known Conc 20 3.01ng/ml 0.112 3.73%
    Inter-assay
    Sample n Mean SD CV%
    Known Conc 10 0.09ng/ml 0.008 8.52%
    Known Conc 10 0.25ng/ml 0.019 7.47%
    Known Conc 10 2.66ng/ml 0.154 5.79%
  • Sample type

    Plasma
  • Assay type

    Quantitative
  • Sensitivity

    0.01 ng/ml
  • Range

    0.01 ng/ml - 10 ng/ml
  • Recovery

    97.75 %

    Sample specific recovery
    Sample type Average % Range
    Spike = 97.7 89.3% - 106%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Sheep
  • Product overview

    Abcam’s uPA Total (URK) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of total urokinase plasminogen activator antigen in mouse plasma.

     

    Mouse uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-murine uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then bound by the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer 1 x 50ml
    Anti-mouse uPA primary antibody 1 vial
    Anti-rabbit horseradish peroxidase secondary antibody 1 vial
    Mouse uPA standard 1 vial
    TMB substrate solution 1 x 10ml
    uPA Antibody pre-coated 96 well microplate (12 x 8 well strips) 1 x 96 tests
  • Research areas

  • Relevance

    Urokinase type plasminogen activator (uPA) is secreted from cells as a precursor which is activated to the two chain form consisting of an A and B chain. The active high MW form is further processed by removal of an amino terminal fragment to an active low MW form (35 kDa). uPA is a serine protease that activates plasminogen toplasmin. High levels of uPA and plasminogen activator inhibitor type 1 (PAI 1) in breast cancer tissue extracts have been associated with rapid disease progression. The malignant phenotype of prostatic tumor cells correlates with the expression of both uPA and its cell membrane receptor (uPAR).
  • Alternative names

    • ATF
    • BDPLT5
    • EC 3.4.21.73
    • PLAU
    • QPD
    • u PA
    • U plasminogen activator
    • u-PA
    • UPA
    • UPAM
    • Urinary plasminogen activator
    • URK
    • Urokinase plasminogen activator
    • Urokinase type plasminogen activator
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab198512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Full range standard curve using ab198512 uPA Total (URK) Mouse ELISA Kit.

  • Linear range standard curve using ab198512 uPA Total (URK) Mouse ELISA Kit.

Protocols

References

ab198512 has not yet been referenced specifically in any publications.

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