Mouse VEGF-B ELISA Kit (ab213897)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 31.2 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
-
Product name
Mouse VEGF-B ELISA Kit
See all VEGFB kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 70pg/ml 4.13 = 5% 2 16 247pg/ml 20.17 = 7.5% 3 16 978pg/ml 48.9 = 5% Inter-assay Sample n Mean SD CV% 1 24 69pg/ml 4.83 = 7% 2 24 246pg/ml 20.17 = 8.2% 3 24 1037pg/ml 59.1 = 5.7% -
Sample type
Cell culture supernatant, Serum, Hep Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 2 pg/ml -
Range
31.2 pg/ml - 2000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse VEGF-B Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213897) is designed for the quantitative detection of Mouse VEGF-B in cell culture supernatants, serum and plasma (heparin).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for VEGF-B has been precoated onto 96-well plates. Standards (Expression system for standard: sf21; Immunogen sequence: Q20-A207 and Q20-R148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF-B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse VEGF-B amount of sample captured in plate.
-
Notes
Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. It is important for the protection of neurons in the retina and the cerebral cortex during stroke and of motor neurons during motor neuron diseases such as amyotrophic lateral sclerosis.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Mouse VEGF-B coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Mouse VEGF-B antibody 1 x 100µl Lyophilized recombinant Mouse VEGF-B standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
-
Function
Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. -
Tissue specificity
Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Post-translational
modificationsVEGF-B186 is O-glycosylated. -
Cellular localization
Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin. - Information by UniProt
-
Alternative names
- Vascular endothelial growth factor B
- Vascular endothelial growth factor-related factor
- VEGF B
see all -
Database links
- Entrez Gene: 22340 Mouse
- SwissProt: P49766 Mouse
- Unigene: 15607 Mouse
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab213897 has been referenced in 3 publications.
- Elhady SS et al. Anticancer Effects of New Ceramides Isolated from the Red Sea Red Algae Hypnea musciformis in a Model of Ehrlich Ascites Carcinoma: LC-HRMS Analysis Profile and Molecular Modeling. Mar Drugs 20:N/A (2022). PubMed: 35049918
- Elhady SS et al. VEGFR-Mediated Cytotoxic Activity of Pulicaria undulata Isolated Metabolites: A Biological Evaluation and In Silico Study. Life (Basel) 11:N/A (2021). PubMed: 34440504
- Llorián-Salvador M et al. VEGF-B Is an Autocrine Gliotrophic Factor for Müller Cells under Pathologic Conditions. Invest Ophthalmol Vis Sci 61:35 (2020). PubMed: 32945843