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The Mouse VEGF-B Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213897) is designed for the quantitative detection of Mouse VEGF-B in cell culture supernatants, serum and plasma (heparin).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for VEGF-B has been precoated onto 96-well plates. Standards (Expression system for standard: sf21; Immunogen sequence: Q20-A207 and Q20-R148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF-B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse VEGF-B amount of sample captured in plate.
Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. It is important for the protection of neurons in the retina and the cerebral cortex during stroke and of motor neurons during motor neuron diseases such as amyotrophic lateral sclerosis.
|Components||1 x 96 tests|
|ABC Diluent Buffer||1 x 12ml|
|Adhesive Plate Seal||4 units|
|Antibody Diluent Buffer||1 x 12ml|
|Anti-Mouse VEGF-B coated Microplate (12 x 8 wells)||1 unit|
|Avidin-Biotin-Peroxidase Complex (ABC)||1 x 130µl|
|Biotinylated anti- Mouse VEGF-B antibody||1 x 130µl|
|Lyophilized recombinant Mouse VEGF-B standard||2 vials|
|Sample Diluent Buffer||1 x 30ml|
|TMB Color Developing Agent||1 x 10ml|
|TMB Stop Solution||1 x 10ml|
Our Abpromise guarantee covers the use of ab213897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab213897 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"