Overview

  • Product name
    Mouse VEGF-B ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    1 16 158pg/ml 6.95 4.4%
    2 16 652pg/ml 33.25 5.1%
    3 16 1309pg/ml 74.61 5.7%
    Inter-assay
    Sample n Mean SD CV%
    1 24 197pg/ml 14.58 7.4%
    2 24 763pg/ml 54.94 7.2%
    3 24 1611pg/ml 124.05 7.7%
  • Sample type
    Cell culture supernatant, Serum, Heparin Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    31.2 pg/ml - 2000 pg/ml
  • Assay time
    3h 30m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    The Mouse VEGF-B Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213897) is designed for the quantitative detection of Mouse VEGF-B in cell culture supernatants, serum and plasma (heparin).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for VEGF-B has been precoated onto 96-well plates. Standards (Expression system for standard: sf21; Immunogen sequence: Q20-A207 and Q20-R148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF-B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse VEGF-B amount of sample captured in plate. 

  • Notes

    Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. It is important for the protection of neurons in the retina and the cerebral cortex during stroke and of motor neurons during motor neuron diseases such as amyotrophic lateral sclerosis.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    ABC Diluent Buffer 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer 1 x 12ml
    Anti-Mouse VEGF-B coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Mouse VEGF-B antibody 1 x 130µl
    Lyophilized recombinant Mouse VEGF-B standard 2 vials
    Sample Diluent Buffer 1 x 30ml
    TMB Color Developing Agent 1 x 10ml
    TMB Stop Solution 1 x 10ml
  • Research areas
  • Function
    Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
  • Tissue specificity
    Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Post-translational
    modifications
    VEGF-B186 is O-glycosylated.
  • Cellular localization
    Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
  • Information by UniProt
  • Alternative names
    • Vascular endothelial growth factor B
    • Vascular endothelial growth factor-related factor
    • VEGF B
    • VEGF related factor
    • VEGF-B
    • VEGF-related factor
    • Vegfb
    • VEGFB_HUMAN
    • VEGFL
    • VRF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse VEGF-B ELISA Kit (ab213897) Standard Curve.

Protocols

References

ab213897 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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