Mouse VEGF ELISA Kit (ab209882)
- Datasheet
- References
- Protocols
Overview
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Product nameMouse VEGF ELISA Kit
See all VEGFA kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall 5 5.6% Inter-assay Sample n Mean SD CV% Overall 3 7.9% -
Sample typeCell culture supernatant, Cell culture extracts, Tissue Extracts
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Assay typeSandwich (quantitative)
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Sensitivity0.3 pg/ml
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Range5.8 pg/ml - 370 pg/ml
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Recovery
Sample specific recovery Sample type Average % Range Cell culture media 109.6 102.9% - 117.1% -
Assay time1h 30m
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Assay durationOne step assay
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Species reactivityReacts with: Mouse
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Product overview
Mouse VEGF in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VEGF protein in mouse cell culture supernatant, cell and tissue extract samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Sensitivity:
Samples diluted in Sample Diluent NS: 0.3 pg/mL
Samples diluted in 1X Cell Extraction Buffer PTR: 0.7 pg/mL
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Notes
VEGF is a secreted growth factor of PDGF family active in angiogenesis, vasculogenesis and endothelial cell growth both in fetus and adult. Alternative splicing produces isoforms including 120, 164 and 188 amino acid long forms in mouse. VEGF induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. VEGF dimers bind to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, induce their homodimerization and autophosphorylation.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate (12 x 8 well strips)
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 10X Mouse VEGF Capture Antibody 1 x 600µl 10X Mouse VEGF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Mouse VEGF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 x 96 tests Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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FunctionGrowth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
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Tissue specificityIsoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
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Involvement in diseaseDefects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
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Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
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Cellular localizationSecreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
- Information by UniProt
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Alternative names
- Vascular endothelial growth factor A
- Vascular permeability factor
- VEGF-A
see all -
Database links
- Entrez Gene: 22339 Mouse
- SwissProt: Q00731 Mouse
Associated products
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Related Products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab209882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Mouse VEGF ELISA Kit (ab209882)ELISA Protocol Summary -
Mouse VEGF standard curve comparison data.Standard curve comparison between mouse VEGF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 9-fold increase in sensitivity.
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Example of mouse VEGF standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of mouse VEGF standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native VEGF in Mouse cell culture supernatant samplesThe concentrations of VEGF were measured in duplicates, interpolated from the VEGFA standard curves and corrected for sample dilution. Undiluted samples are as follows: 5 µg/mL LPS-treated P388D1 Day 6 supernatant 7.5%, lung day 5 supernatant 15%, kidney day 5 supernatant 30%, heart day 5 supernatant 30%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF concentration was determined to be 5,249 pg/mL in LPS P388D1 supernatant, 1,785 pg/mL in lung supernatant, 430 pg/mL in kidney supernatant and 30 pg/mL in heart supernatant samples.
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Interpolated concentrations of native VEGF in mouse lung extract sample based on a 500 µg/mL extract load and in mouse heart extract sample based on a 1,000 µg/mL extract loadThe concentrations of VEGF were measured in duplicate and interpolated from the VEGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGFA concentration was determined to be 842 pg/mL in lung sample and 50 pg/mL in heart sample.
Protocols
References
ab209882 has not yet been referenced specifically in any publications.
