Overview

  • Product name

    Mouse VEGF R3 ELISA Kit (FLT4)
    See all VEGF Receptor 3 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 4.59%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 7.14%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    17.97 pg/ml
  • Range

    46.9 pg/ml - 3000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85 81% - 88%
    Cell culture media 87 87% - 88%
    Heparin Plasma 99 95% - 101%
    EDTA Plasma 100 96% - 104%
    Citrate Plasma 103 101% - 107%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rabbit, Guinea pig, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s VEGF R3 (FLT4) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VEGF R3 protein in mouse serum, plasma, and cell culture supernatants.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    The VEGF receptor family includes three members VEGF R1, VEGF R2, and VEGF R3 (FLT4). These receptors have a characteristic structure that includes 7 Ig-like domains in the extracellular domain and a cytoplasmic tyrosine kinase domain with a long kinase insert region. VEGF R3 is a tyrosine protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD. It plays an essential role in adult lymphangiogenesis, and in the development of the vascular network and cardiovascular system during embryonic development. VEGF R3 promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated VEGF R3 leads to enhanced production of VEGFC and VEGFA, creating a positive feedback loop that enhances FLT4 signaling.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse VEGF R3 Capture Antibody 1 x 600µl
    10X Mouse VEGF R3 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CP 1 x 6ml
    Mouse VEGF R3 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Relevance

    Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine esidues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, and increase vascular permeability in both large and small vessels. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. VEGF Receptor 3 is one of the five receptor tyrosine kinases (RTKs) (VEGF Receptor 1/Flt1, VEGF Receptor 2/KDR/Flk1, VEGF Receptor 3/Flt4, tie1 and tek/tie2) whose expression is almost exclusively restricted to endothelial cells.
  • Cellular localization

    Cell membrane; Single-pass type I membrane protein.
  • Alternative names

    • FLT 4
    • FLT4
    • FLT41
    • Fms like tyrosine kinase 4
    • Fms related tyrosine kinase 4
    • LMPH1A
    • PCL
    • Tyrosine protein kinase receptor FLT4
    • Vascular endothelial growth factor receptor 3
    • VEGFR 3
    • VEGFR3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab203367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The 1X dilution is 5% for mouse serum, and 2.5% for each of the plasmas. Interpolated data values (mean +/- SD, n = 2) are graphed.

  • The 1X dilution is 50.0% for the lung supernatants, and 25.0% for the liver supernatant. Interpolated data values (mean +/- SD, n = 2) are graphed.

Protocols

References

ab203367 has not yet been referenced specifically in any publications.

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