Overview

  • Product name

    Mouse VEGFA ELISA Kit
    See all VEGFA kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 6 3.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 6 6.4%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    20 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75% - 97%
    Serum 87% - 95%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s VEGFA Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse VEGFA in cell culture supernatant, serum and plasma (EDTA).


    Pay attention to a possibly elevated serum level of mouse VEGFA due to VEGFA release by platelets during platelet activation (sampling process).


    VEGFA specific antibodies have been precoated onto 96-well plates. Standards and samples are pipetted into the wells and VEGFA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse VEGFA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGFA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Mouse VEGF ELISA Kit (ab209882) from our SimpleStep ELISA® range.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X conjugate diluent 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 6 units
    Assay Diluent 1 x 12ml
    Biotin-Conjugate anti-mouse VEGFA polyclonal antibody 1 x 120µl
    Microplate coated with polyclonal antibody to Mouse VEGFA (12 x 8 wells) 1 unit
    Sample Diluent 1 x 10ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
    VEGFA Standard lyophilized 2 vials
  • Research areas

  • Function

    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity

    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease

    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization

    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • Alternative names

    • Vascular endothelial growth factor A
    • Vascular permeability factor
    • VEGF-A
    • Vegfa
    • VEGFA_HUMAN
    • VPF
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab119565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119565.

Protocols

References

This product has been referenced in:

  • Meng X  et al. DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma. EBioMedicine 41:185-199 (2019). Read more (PubMed: 30773478) »
  • Park J  et al. VEGF-A-Expressing Adipose Tissue Shows Rapid Beiging and Enhanced Survival After Transplantation and Confers IL-4-Independent Metabolic Improvements. Diabetes 66:1479-1490 (2017). Read more (PubMed: 28254844) »
See all 2 Publications for this product

Customer reviews and Q&As

nice kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
kit worked, easy to use. attached is the data for standard curve. I used cell media from 12 well plate, the media were not concentrated nor diluted, it was directly added to the wells of the kit.

Abcam user community

Verified customer

Submitted Feb 07 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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