Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.98 pg/ml
- Range: 11.72 pg/ml - 750 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat
Product nameMouse/Rat TPA ELISA Kit
See all Tissue Plasminogen Activator kits
Intra-assay Sample n Mean SD CV% Serum 9 3.12% Inter-assay Sample n Mean SD CV% Serum 3 6.88%
Sample typeUrine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range11.72 pg/ml - 750 pg/ml
Sample specific recovery Sample type Average % Range Urine 112 105% - 116% Serum 97 95% - 98% Hep Plasma 100 97% - 101% EDTA Plasma 102 97% - 106% Cit plasma 104 99% - 108%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Mouse, Rat
Does not react with: Cow, Human
Mouse/Rat TPA ELISA Kit (ab233615) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TPA protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat TPA with 4.98 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Mouse/Rat tPA Capture Antibody 1 x 600µl 10X Mouse/Rat tPA Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse/Rat tPA Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionConverts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
Tissue specificitySynthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
Involvement in diseaseNote=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 fibronectin type-I domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain.
DomainBoth FN1 and one of the kringle domains are required for binding to fibrin.
Both FN1 and EGF-like domains are important for binding to LRP1.
The FN1 domain mediates binding to annexin A2.
The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
modificationsThe single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
Characterization of O-linked glycan was studied in Bowes melanoma cell line.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- Plasminogen activator tissue
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The tPA standard curve was prepared as described in Section 10.
The concentrations of tPA were measured in duplicates, interpolated from the tPA standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 12.5%, plasma (EDTA) 12.5%, plasma (citrate) 12.5%, and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean tPA concentration in the neat samples was determined to be 540 pg/mL in serum, 2,567 pg/mL in plasma (heparin), 1,971 pg/mL in plasma (EDTA), 3,141 pg/mL in plasma (citrate), and 2,223 pg/mL in urine.
The concentrations of tPA were measured in duplicates, interpolated from the tPA standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 12.5%, and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean tPA concentration in the neat samples was determined to be 1,657 pg/mL in serum, 5,687 pg/mL in plasma (citrate), and 388 pg/mL in urine.
To learn more about the advantages of recombinant antibodies see here.
ab233615 has not yet been referenced specifically in any publications.