Key features and details
- Mouse monoclonal [15C1B8] to Mov10
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-Mov10 antibody [15C1B8]
See all Mov10 primary antibodies
DescriptionMouse monoclonal [15C1B8] to Mov10
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- Mouse NIH3T3, 293T, HeLa and Jurkat lysate. Human testicular seminoma tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab176687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 114 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceMOV10 may be an helicase with an important function in development and/or control of cell proliferation. RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs). They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs. Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA. Ago1- and Ago2-containing complexes have been purified from human cells, resulting in the discovery of novel factors such as the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage.
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All lanes : Anti-Mov10 antibody [15C1B8] (ab176687) at 0.1 µg/ml
Lane 1 : Mouse NIH-3T3 lysate at 50 µg
Lane 2 : Mouse NIH-3T3 lysate at 15 µg
Lane 3 : 293T lysate at 50 µg
Lane 4 : HeLa lysate at 50 µg
Lane 5 : Jurkat lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 114 kDa
Exposure time: 3 minutes
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testicular seminoma tissue labeling Mov10 with ab176687 at a 1/1000 dilution. DAB staining.
Detection of Human Mov10 by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP Mouse NIH3T3 whole cell lysate loaded. ab176687 used for IP at 6 µg/mg lysate. For blotting immunoprecipitated Mov10, ab176687 was used at 1 µg/ml.
ab176687 has been referenced in 2 publications.
- Puray-Chavez MN et al. Effects of Moloney Leukemia Virus 10 Protein on Hepatitis B Virus Infection and Viral Replication. Viruses 11:N/A (2019). PubMed: 31319455
- Rajgor D et al. NMDAR-dependent Argonaute 2 phosphorylation regulates miRNA activity and dendritic spine plasticity. EMBO J 37:N/A (2018). PubMed: 29712715