• Product name
  • Description
    Rabbit polyclonal to Mov10
  • Host species
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 953 and 1003 of human Mov10 homolog (NP_066014.1)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibody was affinity purified using an epitope specific to MOV10 immobilized on solid support.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab80613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.


  • Relevance
    MOV10 may be an helicase with an important function in development and/or control of cell proliferation. RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs). They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs. Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA. Ago1- and Ago2-containing complexes have been purified from human cells, resulting in the discovery of novel factors such as the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage.
  • Cellular localization
  • Database links
  • Alternative names
    • DKFZp667O1423 antibody
    • FLJ32791 antibody
    • fSAP113 antibody
    • Functional spliceosome-associated protein 113 antibody
    • gb110 antibody
    • MGC2948 antibody
    • Moloney leukemia virus 10 protein antibody
    • MOV 10 antibody
    • Mov10 (Moloney leukemia virus 10, mouse) homolog antibody
    • Mov10 (Moloney leukemia virus 10,) homolog antibody
    • Mov10, Moloney leukemia virus 10, homolog (mouse) antibody
    • Mov10, Moloney leukemia virus 10, homolog antibody
    • Putative helicase MOV10 antibody
    see all


  • All lanes : Anti-Mov10 antibody (ab80613) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 114 kDa
    Observed band size: 114 kDa

    Exposure time: 3 minutes
  • Immunoprecipitation of HeLa whole cell lysate using ab80613 at 3µg/mg (lane 1) or control IgG (lane 2). Samples were analysed by Western blot using ab80613 at 1µg/ml.
    Detection: Chemiluminescence with an exposure time of 30 seconds.
  • ICC/IF image of ab80613 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80613, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Q&A


Thank you for contacting us.

I am sorry to hear of the troubles which you have been experiencing using our products. Could please provide me with details of your western blotting protocol as well as tissue type and preparation steps and any troubleshooting steps which you have performed? After review of these details I may be able to offer recommendations which would improve the results which you have been experiencing. Should we not be able to improve these results, these products are covered by our Abpromise guarantee. We provide replacement or refund should this product not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link:


I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!

Read More


Thank you for your inquiry. Unfortunately, we do not know of a publication where this antibody was used in a co-IP experiment. I would like to reassure you that this antibody is tested and guaranteed for IP by our Abpromise. To co-IP a protein the protocol might have to be adjusted from normal IP. I can recommend to review our Protocol section and the recent webinar about IP from one of our IP experts. Please follow these links: https://www.abcam.com/index.html?pageconfig=resource&rid=11385 http://dev-pws1.abcam.com/blog/index.cfm/2011/11/17/Immunoprecipitation-Procedurespitfalls-and-protocol-tips I hope you will find this information helpful. Please do not hesitate to contact us with any further questions.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up