Immunocytochemistry/ Immunofluorescence abreview for Anti-Mov10 antibody

Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% triton x100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000

Additional data

Additional Notes
HeLa cells were grown on glass coverslips, fixed, permeablized, blocked and stained for Mov10 using ab80613. Discrete cytoplasmic focal staining was observed as shown in the image.

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Submitted Sep 19 2012

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