Overview

  • Product name

    Anti-MOV10L1/Champ antibody
  • Description

    Rabbit polyclonal to MOV10L1/Champ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human MOV10L1/Champ aa 336-425.
    Database link: Q9BXT6

  • Positive control

    • WB: HeLa and PC-3 whole cell lysates. IHC-P: Human breast cancer and liver cancer tissues. ICC/IF: HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab236977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 1 kDa.
IHC-P 1/200 - 1/500.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Putative RNA helicase. Isoform 1 may play a role in male germ cell development.
  • Tissue specificity

    Isoform 1 is specifically expressed in testis.
  • Sequence similarities

    Belongs to the DNA2/NAM7 helicase family. SDE3 subfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cardiac helicase activated by MEF2 protein antibody
    • Cardiac specific RNA helicase antibody
    • CHAMP antibody
    • Csm antibody
    • M10L1_HUMAN antibody
    • Moloney leukemia virus 10 like protein 1 antibody
    • Moloney leukemia virus 10-like protein 1 antibody
    • MOV10 like 1 antibody
    • MOV10 like protein 1 antibody
    • MOV10-like protein 1 antibody
    • MOV10L1 antibody
    • Mov10l1, Moloney leukemia virus 10 like 1, homolog (mouse) antibody
    • Putative helicase Mov10l1 antibody
    see all

Images

  • All lanes : Anti-MOV10L1/Champ antibody (ab236977) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 1 kDa

  • Paraffin-embedded human breast cancer tissue stained for MOV10L1/Champ using ab236977 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human liver cancer tissue stained for MOV10L1/Champ using ab236977 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

           

  • 4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for MOV10L1/Champ (green) using ab236977 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

References

ab236977 has not yet been referenced specifically in any publications.

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