Overview

  • Product name
  • Description
    Goat polyclonal to MPG
  • Host species
    Goat
  • Specificity
    This antibody is expected to recognize all three reported isoforms (NP_002425.2, NP_001015052.1 and NP_001015054.1)
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    SVVDRVAEQDTQA

    , corresponding to C terminal amino acids 286-298 of Human MPG

  • Positive control
    • HEK293 and K562 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 33 kDa).Can be blocked with Human MPG peptide (ab45571).

Target

  • Function
    Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
  • Sequence similarities
    Belongs to the DNA glycosylase MPG family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 alkyladenine DNA glycosylase antibody
    • 3-alkyladenine DNA glycosylase antibody
    • 3-methyladenine DNA glycosidase antibody
    • 3MG_HUMAN antibody
    • AAG antibody
    • ADPG antibody
    • Alkyladenine DNA glycosylase antibody
    • anpg antibody
    • APNG antibody
    • CRA36.1 antibody
    • DNA 3 methyladenine glycosylase antibody
    • DNA-3-methyladenine glycosylase antibody
    • MDG antibody
    • Mid1 antibody
    • Mpg antibody
    • N methylpurine DNA glycosirase antibody
    • N methylpurine DNA glycosylase antibody
    • N-methylpurine-DNA glycosylase antibody
    • PIG11 antibody
    • PIG16 antibody
    • Proliferation inducing protein 11 antibody
    • Proliferation inducing protein 16 antibody
    see all

Images

  • Anti-MPG antibody (ab37983) at 1 µg/ml + HEK293 lysate (RIPA buffer, 35µg total protein per lane).

    Developed using the ECL technique.

    Predicted band size: 33 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    Primary incubated for 1 hour.

References

ab37983 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

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Question
Answer

Sure I can set up a credit for the customer.

If you could, please forward me the customer's email address so I can associate it with the credit note.

Thanks!

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Question
Answer

Thank you for the additional information.

I have initiated the replaplacement request. Please let me know if you have any further questions.

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Question

Inquiry: DETAILS Antibody code:Ab37983
Batch number: GR44142-1 Antibody storage conditions (temperature/reconstitution etc) Aliquots stored at -20C Description of the problem (high background, wrong band size, more bands, no band etc.) Very high background and very low signal for extracts (positive control - ab109955 – nice signal but still high background) Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.) Whole cell extract from purified PBMCs Sample preparation (Buffer/Protease inhibitors/Heating sample etc.) The frozen lymphocytes were thawed at 30°C, after which their protein content was extracted with 220mM KCl for 30 minutes on ice. Cell debris were removed by centrifugation at 13,200 rpm for 15 minutes at 4°C. Glycerol was added to the protein extract to a final concentration of 10%, and the extract was frozen in liquid nitrogen. Amount of protein loaded Up to 40ug Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.) Reducing, 12 and 15 % gel Transfer and blocking conditions (Buffer/time period, Blocking agent etc.) Transfer – Tris / Glycine with MeOH Blocking – TBS-T with 5% milk (I tried also BSA – results were worse) Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Ab37983 – Goat polyclonal 1:400 / 1:1000 ON – 4C / 1hr – RT Wash: 3x 10min / 5x 5min Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Donkey anti goat of two different manufactures (Sigma / Jackson) diluted up to 1:100,000 1hr at RT Wash: 3x 10min / 5x 5min Detection method (ECL, ECLPlus etc.) ECL (also ECL plus that gave even higher background) Positive and negative controls used (please specify) Commercial protein – ab109955
OPTIMIZATION ATTEMPTS (PROBLEM SOLVING) How many times have you tried the Western? I tried a lot of times with extracts from different extractions (from different blood donors), different Ab dilutions, washes, blocking, 2 different second antibodies with a lot of dilution. The background is very very high so I can’t see a signal in extracts only in commercial protein. Have you run a "No Primary" control? No Do you obtain the same results every time? e.g. are the background bands always in the same place? Problem all the time – of course gets worse with lower 2nd Ab dilution etc…. – never got better! What steps have you altered? Extracts source and preparation, Ab dilutions (1st and 2nd), washes, blocking Additional Notes In data shit a nice signal appears – can you give me more details or if you have any suggestions, I’d appreciate it.

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Answer

Thank you for submitting your completed questionnaire. You have done a great job of troubleshooting and it appears the problem in this case is with the antibody.

If you have received this vial of ab37983 within the past 6 months or so I would be happy to offer a replacement.

In your reply, please include the purchase order number or Abcam order reference number associated with this purchase so I can process your request.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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