Anti-MPG/AAG antibody (ab196553)
Key features and details
- Rabbit polyclonal to MPG/AAG
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MPG/AAG antibody
See all MPG/AAG primary antibodies -
Description
Rabbit polyclonal to MPG/AAG -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human MPG/AAG.
Database link: P29372 -
Positive control
- MCF7, K562, U937 and mouse liver lysate. U20S cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 33 kDa.
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Notes |
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IHC-P
1/50 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 33 kDa. |
Target
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Function
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. -
Sequence similarities
Belongs to the DNA glycosylase MPG family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4350 Human
- Entrez Gene: 268395 Mouse
- Omim: 156565 Human
- SwissProt: P29372 Human
- SwissProt: Q04841 Mouse
- Unigene: 459596 Human
- Unigene: 263161 Mouse
- Unigene: 482070 Mouse
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Alternative names
- 3 alkyladenine DNA glycosylase antibody
- 3-alkyladenine DNA glycosylase antibody
- 3-methyladenine DNA glycosidase antibody
see all
Images
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The image on the left is immunohistochemistry of paraffin-embedded Human Lymphoma tissue using ab196553 at dilution 1/40, the image on the right is treated with fusion protein.
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All lanes : Anti-MPG/AAG antibody (ab196553) at 1/950 dilution
Lane 1 : Lovo cell lysate
Lane 2 : PC3 cell lysate
Secondary
All lanes : Goat anti rabbit IgG at 1/8000 dilution
Predicted band size: 33 kDa -
All lanes : Anti-MPG/AAG antibody (ab196553) at 1/500 dilution
Lane 1 : MCF7 extract
Lane 2 : K562 extract
Lane 3 : U937 extract
Lane 4 : Mouse liver extract
Predicted band size: 33 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab196553 has been referenced in 1 publication.
- Ding X et al. The role of H3K9me2-regulated base excision repair genes in the repair of DNA damage induced by arsenic in HaCaT cells and the effects of Ginkgo biloba extract intervention. Environ Toxicol 36:850-860 (2021). PubMed: 33378118