Overview

  • Product name

    Anti-MPG/AAG antibody [EPR10959(B)]
    See all MPG/AAG primary antibodies
  • Description

    Rabbit monoclonal [EPR10959(B)] to MPG/AAG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human MPG/AAG.
    Database link: P29372

  • Positive control

    • HeLa, 293T and Jurkat cell lysates; Human testis tissue and ovarian carcinoma tissue.
  • General notes

     This product was previously labelled as MPG

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10959(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.

Use with paraformaldehyde fixed cells, permeabilized with 0.5% Triton X100/PBS.

WB 1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
  • Sequence similarities

    Belongs to the DNA glycosylase MPG family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 alkyladenine DNA glycosylase antibody
    • 3-alkyladenine DNA glycosylase antibody
    • 3-methyladenine DNA glycosidase antibody
    • 3MG_HUMAN antibody
    • AAG antibody
    • ADPG antibody
    • Alkyladenine DNA glycosylase antibody
    • anpg antibody
    • APNG antibody
    • CRA36.1 antibody
    • DNA 3 methyladenine glycosylase antibody
    • DNA-3-methyladenine glycosylase antibody
    • MDG antibody
    • Mid1 antibody
    • Mpg antibody
    • N methylpurine DNA glycosirase antibody
    • N methylpurine DNA glycosylase antibody
    • N-methylpurine-DNA glycosylase antibody
    • PIG11 antibody
    • PIG16 antibody
    • Proliferation inducing protein 11 antibody
    • Proliferation inducing protein 16 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MPG/AAG knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab155092 observed at 35 kDa. Red - loading control, ab8245.

    ab155092 was shown to specifically react with MPG/AAG when MPG/AAG knockout samples were used. Wild-type and MPG/AAG knockout samples were subjected to SDS-PAGE.  Ab155092 and ab8245 (loading control to GAPDH) were diluted at 1/10,000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155092 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • All lanes : Anti-MPG/AAG antibody [EPR10959(B)] (ab155092) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 33 kDa

  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MPG/AAG with unpurified ab155092 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MPG/AAG with ab155092 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human tesis tissue labeling MPG/AAG with ab155092 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue using ab155092 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155092 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155092 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab155092 staining MPG/AAG in human primary fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • ab155092 (1/500) staining MPG/AAG in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5%Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

References

This product has been referenced in:

  • Venkatachalam G  et al. Replication stress-induced endogenous DNA damage drives cellular senescence induced by a sub-lethal oxidative stress. Nucleic Acids Res 45:10564-10582 (2017). Read more (PubMed: 28985345) »
  • Khoronenkova SV & Dianov GL ATM prevents DSB formation by coordinating SSB repair and cell cycle progression. Proc Natl Acad Sci U S A 112:3997-4002 (2015). WB ; Human . Read more (PubMed: 25775545) »
See all 3 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.3% Triton-X100 in blocking buffer
Specification
HEK293
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 17 2018

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (fibroblast)
Total protein in input
500 µg
Immuno-precipitation step
Protein A
Specification
fibroblast

Abcam user community

Verified customer

Submitted Oct 19 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (Lymphocytes)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
30 µg
Specification
Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 14 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human primary fibroblasts)
Permeabilization
Yes - 0.2% Triton-X100
Specification
Human primary fibroblasts
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 26 2014

Application
Western blot
Sample
Human Cell lysate - whole cell (TIG-1 primary fibroblasts)
Gel Running Conditions
Reduced Denaturing (4-16% gradient Tris-Gly gel)
Loading amount
30 µg
Specification
TIG-1 primary fibroblasts
Blocking step
(agent) for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Sep 04 2013

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-16% gradient Tris-Gly gel)
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification
Mouse embryonic fibroblasts
Blocking step
LI-COR­ Odyssey­ Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Sep 04 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Feb 19 2013

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