Anti-MPP1 antibody (ab241086)
Key features and details
- Rabbit polyclonal to MPP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MPP1 antibody
See all MPP1 primary antibodies -
Description
Rabbit polyclonal to MPP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MPP1 aa 1-50. The exact sequence is proprietary.
Database link: Q00013 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: MPP1 IP in Jurkat whole cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000.
|
|
IP |
Use at 2-10 µg/mg of lysate.
|
Notes |
---|
WB
1/1000 - 1/5000. |
IP
Use at 2-10 µg/mg of lysate. |
Target
-
Function
Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 1 PDZ (DHR) domain.
Contains 1 SH3 domain. -
Post-translational
modificationsExtensively palmitoylated. -
Cellular localization
Membrane. Cell projection > stereocilium. Colocalizes with WHRN at stereocilium tip during hair cell development (By similarity). Colocalizes with MPP5 in the retina, at the outer limiting membrane (OLM). Colocalizes with WHRN in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies and at the connecting cilium (CC). Colocalizes with NF2 in non-myelin-forming Schwann cells. - Information by UniProt
-
Database links
- Entrez Gene: 4354 Human
- Omim: 305360 Human
- SwissProt: Q00013 Human
- Unigene: 496984 Human
-
Alternative names
- 55 kDa erythrocyte membrane protein antibody
- AAG 12 antibody
- AAG12 antibody
see all
Images
-
MPP1 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP; 20% of IP loaded prepared using RIPA buffer) using ab241086 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab241086 at 1 µg/mL.
Lane 1: ab241086 IP in Jurkat whole cell lysate.
Lane 2: Control IgG.Detection: Chemiluminescence with an exposure time of 3 minutes.
-
All lanes : Anti-MPP1 antibody (ab241086) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutesLysates prepared using RIPA buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab241086 has not yet been referenced specifically in any publications.