Anti-MPP1 antibody (ab96255)
Key features and details
- Rabbit polyclonal to MPP1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MPP1 antibody
See all MPP1 primary antibodies -
Description
Rabbit polyclonal to MPP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Cow
-
Immunogen
Recombinant fragment containing a sequence corresponding to a region within amino acids 214-398 of Human MPP1 (NP_002427).
-
Positive control
- HeLa whole cell lysate, Cal27 xenograft; 293T, A431, H1299, HepG2, Molt-4 and Raji cell lysates
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab96255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 52 kDa. | |
| IHC-P | 1/100 - 1/500. |
Target
-
Function
Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 1 PDZ (DHR) domain.
Contains 1 SH3 domain. -
Post-translational
modificationsExtensively palmitoylated. -
Cellular localization
Membrane. Cell projection > stereocilium. Colocalizes with WHRN at stereocilium tip during hair cell development (By similarity). Colocalizes with MPP5 in the retina, at the outer limiting membrane (OLM). Colocalizes with WHRN in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies and at the connecting cilium (CC). Colocalizes with NF2 in non-myelin-forming Schwann cells. - Information by UniProt
-
Database links
- Entrez Gene: 510998 Cow
- Entrez Gene: 4354 Human
- Entrez Gene: 17524 Mouse
- Entrez Gene: 100328768 Rabbit
- Omim: 305360 Human
- SwissProt: Q17QN6 Cow
- SwissProt: Q00013 Human
- SwissProt: P70290 Mouse
see all -
Alternative names
- 55 kDa erythrocyte membrane protein antibody
- AAG 12 antibody
- AAG12 antibody
see all
Images
-
Western blot - Anti-MPP1 antibody (ab96255)Anti-MPP1 antibody (ab96255) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 52 kDa
10% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP1 antibody (ab96255)Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ab96255 antibody at 1/100 dilution.
Protocols
References (1)
ab96255 has been referenced in 1 publication.
- Huang NJ et al. Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin. Nat Commun 8:423 (2017). PubMed: 28871080
