Anti-MPP1 antibody (ab96255)

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Western blot - Anti-MPP1 antibody (ab96255)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP1 antibody (ab96255)

Key features and details

  • Rabbit polyclonal to MPP1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-MPP1 antibody
    See all MPP1 primary antibodies

  • Description

    Rabbit polyclonal to MPP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow

  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 214-398 of Human MPP1 (NP_002427).

  • Positive control

    • HeLa whole cell lysate, Cal27 xenograft; 293T, A431, H1299, HepG2, Molt-4 and Raji cell lysates

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab96255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P
1/100 - 1/500.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P
1/100 - 1/500.

Target

  • Function

    Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SH3 domain.

  • Post-translational
    modifications

    Extensively palmitoylated.

  • Cellular localization

    Membrane. Cell projection > stereocilium. Colocalizes with WHRN at stereocilium tip during hair cell development (By similarity). Colocalizes with MPP5 in the retina, at the outer limiting membrane (OLM). Colocalizes with WHRN in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies and at the connecting cilium (CC). Colocalizes with NF2 in non-myelin-forming Schwann cells.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 55 kDa erythrocyte membrane protein antibody
    • AAG 12 antibody
    • AAG12 antibody
    • Aging associated gene 12 antibody
    • DXS552 antibody
    • DXS552E antibody
    • EM55_HUMAN antibody
    • EMP 55 antibody
    • EMP55 antibody
    • Erythrocyte membrane protein p55 antibody
    • Membrane protein antibody
    • Membrane protein palmitoylated 1 55kDa antibody
    • Membrane protein palmitoylated 1 antibody
    • Membrane protein, palmitoylated 1, 55kDa antibody
    • Migration related gene 1 antibody
    • MPP 1 antibody
    • MPP1 antibody
    • MRG 1 antibody
    • MRG1 antibody
    • OTTHUMP00000026054 antibody
    • OTTHUMP00000196176 antibody
    • p55 antibody
    • palmitoylated 1 antibody
    • Palmitoylated erythrocyte membrane protein antibody
    • Palmitoylated membrane protein 1 antibody
    • PEMP antibody
    see all

Images

  • Western blot - Anti-MPP1 antibody (ab96255)
    Western blot - Anti-MPP1 antibody (ab96255)
    Anti-MPP1 antibody (ab96255) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 52 kDa



    10% SDS-PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP1 antibody (ab96255)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP1 antibody (ab96255)
    Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ab96255 antibody at 1/100 dilution.

References (1)

Publishing research using ab96255? Please let us know so that we can cite the reference in this datasheet.

ab96255 has been referenced in 1 publication.

  • Huang NJ  et al. Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin. Nat Commun 8:423 (2017). PubMed: 28871080

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