Overview

  • Product name

    Anti-MPP1 antibody [EPR5864]
    See all MPP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5864] to MPP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MPP1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, Jurkat, Human heart, and K562 cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.52 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5864
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the MAGUK family.
      Contains 1 guanylate kinase-like domain.
      Contains 1 PDZ (DHR) domain.
      Contains 1 SH3 domain.
    • Post-translational
      modifications

      Extensively palmitoylated.
    • Cellular localization

      Membrane. Cell projection > stereocilium. Colocalizes with WHRN at stereocilium tip during hair cell development (By similarity). Colocalizes with MPP5 in the retina, at the outer limiting membrane (OLM). Colocalizes with WHRN in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies and at the connecting cilium (CC). Colocalizes with NF2 in non-myelin-forming Schwann cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • 55 kDa erythrocyte membrane protein antibody
      • AAG 12 antibody
      • AAG12 antibody
      • Aging associated gene 12 antibody
      • DXS552 antibody
      • DXS552E antibody
      • EM55_HUMAN antibody
      • EMP 55 antibody
      • EMP55 antibody
      • Erythrocyte membrane protein p55 antibody
      • Membrane protein antibody
      • Membrane protein palmitoylated 1 55kDa antibody
      • Membrane protein palmitoylated 1 antibody
      • Membrane protein, palmitoylated 1, 55kDa antibody
      • Migration related gene 1 antibody
      • MPP 1 antibody
      • MPP1 antibody
      • MRG 1 antibody
      • MRG1 antibody
      • OTTHUMP00000026054 antibody
      • OTTHUMP00000196176 antibody
      • p55 antibody
      • palmitoylated 1 antibody
      • Palmitoylated erythrocyte membrane protein antibody
      • Palmitoylated membrane protein 1 antibody
      • PEMP antibody
      see all

    Images

    • All lanes : Anti-MPP1 antibody [EPR5864] (ab133500) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Human heart lysate
      Lane 5 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 52 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133500 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133500.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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