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    mpp2dlg2-antibody-ab97290.pdf

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Signal Transduction Adapters Transmembrane
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Anti-MPP2/DLG2 antibody (ab97290)

  • Datasheet
  • SDS
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Western blot - Anti-MPP2/DLG2 antibody (ab97290)

    Key features and details

    • Rabbit polyclonal to MPP2/DLG2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-MPP2/DLG2 antibody
      See all MPP2/DLG2 primary antibodies
    • Description

      Rabbit polyclonal to MPP2/DLG2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat
    • Immunogen

      Recombinant fragment corresponding to Human MPP2/DLG2 aa 112-432. UniProt ID: Q14168 isoform 2
      Database link: NP_005365

    • Positive control

      • A549 whole cell lysate; HepG2 and HCT116 cell lysates
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Adapters
      • Transmembrane

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • A549 whole cell lysate (ab7910)

    Applications

    Our Abpromise guarantee covers the use of ab97290 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/3000. Predicted molecular weight: 65 for isoform 1 , for isoform 2, 62 kDa.

    Target

    • Relevance

      MPP2/DLG2 (Palmitoylated membrane protein 2) is a member of a family of membrane associated proteins termed MAGUKs (membrane associated guanylate kinase homologs). These proteins interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP2/DLG2 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction.
    • Cellular localization

      Cell Membrane; integral membrane protein
    • Database links

      • Entrez Gene: 4355 Human
      • Entrez Gene: 50997 Mouse
      • Entrez Gene: 85275 Rat
      • Omim: 600723 Human
      • SwissProt: Q14168 Human
      • SwissProt: Q9WV34 Mouse
      • Unigene: 514208 Human
      • Unigene: 36242 Mouse
      • Unigene: 16404 Rat
      see all
    • Alternative names

      • D11BWG0652E antibody
      • Discs Large Homolog 2 antibody
      • DLG2 antibody
      • DLGH2 antibody
      • Homolog of DLG A tumor supressor antibody
      • MAGUK p55 Subfamily Member 2 antibody
      • Membrane Protein Palmitoylated 2 antibody
      • MPP2_HUMAN antibody
      • PALS4 antibody
      see all

    Images

    • Western blot - Anti-MPP2/DLG2 antibody (ab97290)
      Western blot - Anti-MPP2/DLG2 antibody (ab97290)
      Anti-MPP2/DLG2 antibody (ab97290) at 1/1000 dilution + A549 whole cell lysate at 30 µg

      Predicted band size: 65 for isoform 1 , for isoform 2, 62 kDa



      7.5% SDS PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab97290? Please let us know so that we can cite the reference in this datasheet.

    ab97290 has been referenced in 2 publications.

    • Lai XL  et al. Apc gene suppresses intracranial aneurysm formation and rupture through inhibiting the NF-?B signaling pathway mediated inflammatory response. Biosci Rep 39:N/A (2019). PubMed: 30808715
    • Rademacher N  et al. MPP2 is a postsynaptic MAGUK scaffold protein that links SynCAM1 cell adhesion molecules to core components of the postsynaptic density. Sci Rep 6:35283 (2016). WB, ICC/IF . PubMed: 27756895

    Customer reviews and Q&As

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