Product nameAnti-MPP3/DLG3 antibody
See all MPP3/DLG3 primary antibodies
DescriptionRabbit polyclonal to MPP3/DLG3
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human stomach tissue. ICC/IF: U-251 MG cells. WB: MPP3/DLG3 over expressed HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.4 µg/ml. Predicted molecular weight: 24 kDa.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 2 L27 domains.
Contains 1 PDZ (DHR) domain.
Contains 1 SH3 domain.
- Information by UniProt
- Discs large (Drosophila) homolog 3 antibody
- Discs large homolog 3 antibody
- DLG 3 antibody
Paraffin-embedded human stomach tissue stained for MPP3/DLG3 using ab254634 at 1/20 dilution in immunohistochemical analysis.
Lane 2 : Anti-MPP3/DLG3 antibody (ab254634) at 0.4 µg/ml
Lane 1 : Control
Lane 2 : MPP3/DLG3 overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Predicted band size: 24 kDa
PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling MPP3/DLG3 using ab254634 at 4µg/ml (green) in ICC/IF.
ab254634 has not yet been referenced specifically in any publications.