Product nameAnti-MPP5 antibody - N-terminal
See all MPP5 primary antibodies
DescriptionRabbit polyclonal to MPP5 - N-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Synthetic peptide, corresponding to a region within N-terminal amino acids 124217 of Human MPP5 (Uniprot ID Q8N3R9).
- Recombinant Human MPP5 protein (ab132220) can be used as a positive control in WB. U87-MG and SK-N-SH whole cell lysates, HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 77 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionMay play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton.
Tissue specificityExpressed in the retina (at protein level).
Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 2 L27 domains.
Contains 1 PDZ (DHR) domain.
Contains 1 SH3 domain.
DomainThe L27 domain 1 functions in targeting to the tight junctions by binding to INADL.
The PDZ domain binds to the C-terminus of SC6A1.
Cellular localizationCell membrane. Endomembrane system. Cell junction > tight junction. Localized to the tight junctions of epithelial cells and a subset of intracellular vesicles. In the retina, detected at the outer limiting membrane (OLM), apical to the adherens junction (AJ), where it colocalizes with CRB1. Colocalizes with MPP1 in the retina at the outer limiting membrane (OLM). Localized to the Purkinje cell body and axon.
- Information by UniProt
- FLJ12615 antibody
- MAGUK p55 subfamily member 5 antibody
- Membrane protein palmitoylated 5 antibody
All lanes : Anti-MPP5 antibody - N-terminal (ab155132) at 1/500 dilution
Lane 1 : U87-MG whole cell lysate
Lane 2 : SK-N-SH whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 77 kDa
7.5% SDS PAGE
Immunofluorescent analysis of methanol-fixed heLa cells labeling MPP5 with ab155132 at 1/500. Alpha-tubulin filaments were labeled red.
ab155132 has not yet been referenced specifically in any publications.