Overview

  • Product name
    Anti-MPP5 antibody - N-terminal
    See all MPP5 primary antibodies
  • Description
    Rabbit polyclonal to MPP5 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide, corresponding to a region within N-terminal amino acids 124217 of Human MPP5 (Uniprot ID Q8N3R9).

  • Positive control
    • Recombinant Human MPP5 protein (ab132220) can be used as a positive control in WB. U87-MG and SK-N-SH whole cell lysates, HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab155132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 77 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    May play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton.
  • Tissue specificity
    Expressed in the retina (at protein level).
  • Sequence similarities
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 2 L27 domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SH3 domain.
  • Domain
    The L27 domain 1 functions in targeting to the tight junctions by binding to INADL.
    The PDZ domain binds to the C-terminus of SC6A1.
  • Cellular localization
    Cell membrane. Endomembrane system. Cell junction > tight junction. Localized to the tight junctions of epithelial cells and a subset of intracellular vesicles. In the retina, detected at the outer limiting membrane (OLM), apical to the adherens junction (AJ), where it colocalizes with CRB1. Colocalizes with MPP1 in the retina at the outer limiting membrane (OLM). Localized to the Purkinje cell body and axon.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ12615 antibody
    • MAGUK p55 subfamily member 5 antibody
    • Membrane protein palmitoylated 5 antibody
    • MPP 5 antibody
    • MPP5 antibody
    • MPP5_HUMAN antibody
    • PALS 1 antibody
    • PALS1 antibody
    • Protein associated with Lin-7 antibody
    • Stardust (Drosophila) antibody
    • Stardust antibody
    see all

Images

  • All lanes : Anti-MPP5 antibody - N-terminal (ab155132) at 1/500 dilution

    Lane 1 : U87-MG whole cell lysate
    Lane 2 : SK-N-SH whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 77 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed heLa cells labeling MPP5 with ab155132 at 1/500. Alpha-tubulin filaments were labeled red.

References

ab155132 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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